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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1968

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
The acute toxicity of 4 solid, crystalline xylenol isomers including 2,5-xylenol was studied in white mice, albino rats, and rabbits. A single dose of the substances was administered by mouth in the form of finely dispersed aqueous suspensions. The animals were kept under clinical observation for 15 days. Data on rat and mice mortality were processed statistically by probit analysis as modified by Prozorovsky (1962). LD50 for rabbits was determined by the method of Deichmann and Le Blanc (1943).
GLP compliance:
no
Test type:
other: not specified
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: crystalline

Test animals

Species:
rat
Strain:
other: albino rats
Sex:
not specified

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
water
Details on oral exposure:
A single dose of 2,5-xylenol was administered by mouth in the form of a finely dispersed aqueous suspension of the solid, cristalline material.
Doses:
not specified
No. of animals per sex per dose:
not specified
Control animals:
not specified
Details on study design:
The acute toxicity of 2,5-xylenol was studied in white mice, albino rats, and rabbits. A single dose of the substance was administered by mouth in the form of a finely dispersed aqueous suspension of the solid, cristalline material. The animals were kept under clinical observation for 15 days.
Statistics:
Mortality data of rats and mice were processed statistically by probit analysis as modified by Prozorovsky; LD50 for rabbits was determined by the method of Deichmann and Le Blanc (1943).

References:

Deichmann W, LeBlanc T (1943) Determination of the approximate lethal dose with about 6 animals. J Ind Hyp Toxicol 25, 415-417.
Prozorovsky VB (1962) Application of a method of least squares for probit-analysis of the lethality curves // Farmakol. i toksikol. - 1962. -N9l. - P. 115-120 (in Russian).

Results and discussion

Effect levels
Key result
Sex:
not specified
Dose descriptor:
LD50
Remarks:
species: rat
Effect level:
ca. 444 mg/kg bw
Based on:
test mat.
Remarks on result:
other:
Remarks:
S:E. 26 mg/kg bw
Mortality:
Most of the animals with acute poisoning died within 24 hours of administration.
Clinical signs:
The clinical picture was similar in all the species of laboratory animals investigated (and for all of the 4 isomers considered). The clinical signs of acute poisoning were dyspnea, disturbance of motor coordination, a rapid advent of clonic spasms, and an asymmetrical body position.
Body weight:
not specified
Gross pathology:
no data

Any other information on results incl. tables

Species

LD50-values (mg/kg bw ± standard error if indicated)

White mice

383 ± 36

Albino rats

444 ± 26

Rabbits

938

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
2,5-Xylenol showed slight acute oral toxicity, with a rat LD50 value of 444 ± 26 (S.E.) mg per kg body weight.
Executive summary:

The acute toxicity of 4 solid, crystalline xylenol isomers including 2,5-xylenol was studied in white mice, albino rats, and rabbits in accordance with international accepted scientific methods. No guideline was followed. A single dose of the substances was administered by mouth in the form of finely dispersed aqueous suspensions. The animals were kept under clinical observation for 15 days. Data on rat and mice mortality were processed statistically by probit analysis as modified by Prozorovsky (1962).  LD50 for rabbits was determined by the method of Deichmann and Le Blanc (1943). The clinical picture was similar in all the species of laboratory animals investigated (and for all the isomers considered). The clinical signs of acute poisoning were dyspnea, disturbance of motor coordination, a rapid advent of clonic spasms, and an asymmetrical body position. Most of the animals with acute poisoning died within 24 hours of administration.

Based on the study results, LD50values of 383 ±36 (S.E.), 444 ± 26, and 938 mg/kg bw were calculated for white mice, albino rats and rabbits, respectively.