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EC number: 701-083-2
CAS number: -
the results of the test.
No significant inhibition of respiration rate
of the sludge was recorded at 100 mg
1:3 per litre. The duplicate measurement confirmed the result of the
first measurement. Therefore, no further testing was needed.
Hence, the EC50of Polyborate 1:3
exceeded 100 mg/l (based on nominal concentrations).
(C) and Polyborate 1:3 (T): pH, oxygen concentration, oxygen consumption
and percentage inhibitionof the
at the start
¹: A negative value indicates
stimulation of the respiration rate. This value is considered as not
The influence of MEA Polyborate 1:3 on the
respiration rate of activated sludge was investigated after a contact
time of 3 hours.
The study procedures described in this report
were based onthe OECD guideline No.
addition, the procedures were designed to meet the test methods of theCommissionRegulation
(EC) No. 440/2008 of 30 May 2008,
Publication No. L142, Part C11 and ISO Standard 8192 (2007).
The batch of MEA Polyborate 1:3 tested was aclear
colourless liquid.MEA Polyborate 1:3
was tested as delivered by the sponsor.
A stock solution of 0.5 g/l was prepared by
adding 0.1255 g test substance to 250 ml of
Milli-RO water. Thorough mixing (14 minutes)
was used to accelerate dissolution and to ensure homogeneity. The
synthetic sewage feed (16 ml), activated sludge (200 ml) and an adequate
amount of the clear colourless test substance stock solution were mixed
and made up to 500 ml with Milli-RO water in a 1-litre bottle. A
concentration of 100 mg/l was tested in duplicate.
Two controls without test substance were
tested in each test series.
Optimal contact between the test substance
and test medium was ensured applying continuous aeration and stirring
during the 3-hour exposure period. Thereafter, oxygen consumption was
measured and recorded for approximately 10 minutes.
MEA Polyborate 1:3 per litre. The duplicate
measurement confirmed the result of the first measurement. Therefore, no
further testing was needed.
Hence, the EC50of MEA Polyborate
1:3 exceeded 100 mg/l (based on nominal concentrations).
batch of activated sludge was checked for sensitivity by testing the
Since all criteria for acceptability of the
test were met, this study was considered to be valid.
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