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EC number: 701-083-2
CAS number: -
ThCO2, expressed as mg CO2/mg test substance, was calculated from the
results of carbon analysis. The first step in calculating the amount of
CO2 produced is to correct for background (endogenous) CO2 production.
Thus the amount of CO2 produced by a test substance is determined by the
difference (in ml of titrant) between the experimental and blank Ba(OH)2
traps. The amount of 0.05 N HCl titrated is converted into mg of CO2
produced: 44 1.1 ml HCl titrated 2 0.05 ml HCl titrated mg CO2 Relative
biodegradation values were calculated from the cumulative CO2 production
relative to the total expected CO2 production based on the total carbon
content of the amount of test substance present in the test bottles. A
figure of more than 10% biodegradation was considered significant. The
relative biodegradation values were plotted versus time together with
the relative biodegradation of the positive control. If applicable, the
number of days is calculated from the attainment of 10% biodegradation
until 60% biodegradation. Should this period be ≤ 10 days (10-day
window), then the test substance is designated as readily biodegradable.
Toxicity control: if less than 25% biodegradation (based on ThCO2)
occurred within 14 days, the test substance was assumed to be
inhibitory. The total CO2 evolution in the inoculum blank was determined
by the cumulative difference (in ml of titrant) between the blank
Ba(OH)2 traps and fresh Ba(OH)2.
Determination of ‘ready’ biodegradability:
carbon dioxide (CO2) evolution test (modified Sturm test)
with MEA Polyborate 1:1.
The study procedures described in this report
were based on the OECD guideline No. 301 B, 1992. In addition, the
procedures were designed to meet the test methods of theCommissionRegulation
(EC) No. 440/2008 of 30 May 2008, Publication No.L142,
Part C.4-C and the ISO International Standard 9439, 1999.
The batch of MEA Polyborate 1:1 tested was aclear
colourless liquid.MEA Polyborate 1:1 was
tested as delivered by the sponsor. The organic carbon content ofMEA
Polyborate 1:1could not be calculated and therefore the Total
Organic Carbon (TOC) content was determined.
The TOC content ofMEA Polyborate 1:1was
determined to be 14.42%.MEA Polyborate 1:1was
tested in duplicate at 136.5 mg/l, corresponding to 20 mg TOC/l.Based
on the TOC content the ThCO2of MEA Polyborate 1:1 was
calculated to be 0.53 mg CO2/mg.
The study consisted of six bottles:
inoculum blanks (no test substance),
test bottles (MEA Polyborate 1:1),
positive control (sodium acetate) and
toxicity control (MEA Polyborate 1:1 plus sodium acetate).
Weighed amounts of MEA Polyborate 1:1 were
added to the 2-litres test bottles containing medium with microbial
organisms and mineral components. To this end, 20 ml of Milli-RO water
was added to each weighing bottle containing the test substance. After
vigorous mixing (vortex) the resulting suspension was added
quantitatively to the test medium. The test solutions were continuously
stirred during the test, to ensure optimal contact between the test
substance and the test organisms. Test duration was 28 days (last CO2-measurement
on the 29thday).
The relative biodegradation values calculated
from the measurements performed during the test period revealed 69 and
77% biodegradation of MEA Polyborate 1:1 for A and B, respectively.
Furthermore, in test bottle B biodegradation
of MEA Polyborate 1:1 of at least 60% was reached within a 10-day window.
In the toxicity control, MEA Polyborate 1:1
was found not to inhibit microbial activity.
Since all criteria for acceptability of the
test were met, this study was considered to be valid.
In conclusion, MEA Polyborate 1:1 is
designated as readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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