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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
data is from NTRL reports

Data source

Reference
Reference Type:
secondary source
Title:
LETTER CONCERNING FIVE ENCLOSED STUDIES ON 2-ETHYLANTHRAQUINONE WITH ATTACHMENTS (SANITIZED)
Author:
NTRL Report
Year:
1991
Bibliographic source:
NTRL Report: OTS0534314; last updated 1991

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
The skin irritation potential of 2-ethylanthraquinone (CAS No: 84-51-5)was evaluated in albino rabbits according to the Draize method
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
Name of the test chemical: 2-ethylanthraquinone
Molecular Formula: C16H12O2
Molecular Weight: 236.316 g/mol
SMILES: c12c(C(c3ccccc3C1=O)=O)ccc(c2)CC
InChI: 1S/C16H12O2/c1-2-10-7-8-13-14(9-10)16(18)12-6-4-3-5-11(12)15(13)17/h3-9H,2H2,1H3
Substance type: Organic
Physical State: Solid
Specific details on test material used for the study:
Name of the test chemical: 2-ethylanthraquinone
Molecular Formula: C16H12O2
Molecular Weight: 236.316 g/mol
SMILES: c12c(C(c3ccccc3C1=O)=O)ccc(c2)CC
InChI: 1S/C16H12O2/c1-2-10-7-8-13-14(9-10)16(18)12-6-4-3-5-11(12)15(13)17/h3-9H,2H2,1H3
Substance type: Organic
Physical State: Solid

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals and environmental conditions:
Test Animals:
- Sex: male and female

Test system

Type of coverage:
occlusive
Preparation of test site:
other: intact and abraded
Vehicle:
unchanged (no vehicle)
Controls:
not specified
Amount / concentration applied:
0.5 ml (0.5g)
Duration of treatment / exposure:
24 hours
Observation period:
24 and 72 hours
Number of animals:
6 (3 male, 3 female)
Details on study design:
TEST SITE
- Area of exposure: trunk,
- % coverage:1 inch *1 inch
- Type of wrap if used: an impermeable occlusive wrapping

REMOVAL OF TEST SUBSTANCE
- Washing (if done):no
- Time after start of exposure:

SCORING SYSTEM: The sites were individually examined and scored separately for erythema and edema at 24 and 72 hours. The mean scores for 24 and 72 hours grading were averaged to determine final irritation indices.

Results and discussion

In vitro

Other effects / acceptance of results:
no data available

In vivo

Results
Irritation parameter:
primary dermal irritation index (PDII)
Basis:
mean
Time point:
other: average of 24 and 72 hours
Score:
0.15
Max. score:
8
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
The Primary Irritation Index after 24, 72 hours for 2-ethylanthraquione was 0.15.

Any other information on results incl. tables

Table 3: Primary Skin Irritation – Rabbits

Summary of Scores for skin irritation

Rabbit number

Skin condition

Hours

24

 

72

E1

Ed1

E1

Ed1

1

NA2

0

0

0

0

A3

0

0

0

0

2

NA

0

0

0

0

A

0

0

0

0

3

NA

1

0

0

0

A

1

0

0

0

4

NA

0

0

0

0

A

0

0

0

0

5

NA

0

0

0

0

A

0

0

0

0

6

NA

1

0

0

0

A

1

0

0

0

Average

NA

0.3

0

0

0

A

0.3

0

0

0

Combined Averages = 0.6           

Primary Irritation Index = 0.15

Where,

1- E/Ed = Erythema and Edema 

2- NA = Non- abraded skin

3- A = Abraded skin

Applicant's summary and conclusion

Interpretation of results:
other: not irritating
Conclusions:
The Primary Irritation Index after 24, 72 hours for 2-ethylanthraquinone was 0.15.
Based on the score and scale of interpretation of PII scores, 2-Ethylanthraquinone (CAS No: 84-51-5) can be considered not irritating to skin.
Executive summary:

The skin irritation potential of 2-ethylanthraquinone (CAS No: 84-51-5) was evaluated in albino rabbits according to the Draize method. 6 albino New Zealand rabbits (3/sex) were used for the study. 0.5 ml (0.5 g) of the test material was applied to clipped areas of intact and abraded skin.

The abrasions were longitudinal epidermal incisions sufficiently deep to penetrate the stratum corneum, but not so deep as to destroy the integrity of the derma. Applications were made under occlusive patches (1" x 1" gauze, covered by adhesive tape). Following application of 2-ethylanthraquinone the entire trunk of each animal was covered with an impermeable occlusive wrapping. The animals were then immobilized. The wrapping and test material were removed 24 hours following application. The sites were individually examined and scored separately for erythema and edema at 24 and 72 hours. The mean scores for 24 and 72 hours grading were averaged to determine final irritation indices.

 The Primary Irritation Index after 24, 72 hours for 2-ethylanthraquinone was 0.15. Based on the score and scale of interpretation of PII scores, 2-Ethylanthraquinone (CAS No: 84-51-5) can be considered not irritating to skin.