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EC number: 701-120-2
CAS number: -
OECD 202, GLP, Klimisch 1. Daphnia acute 48 hour EC50 5.18 mg/L.
The acute toxicity of the test item OLEIC_DimerFA_TETA_PAA (batch no.:
BB 100860 V1) to Daphnia magna was determined according to
OECD-Guideline for Testing of Chemicals No. 202 (2004) at
Dr.U.Noack-Laboratorien, 31157 Sarstedt, Germany. the study was
conducted in natural river water.
A static test was performed with the nominal concentrations of 0.625 -
1.25 - 2.50 - 5.00 - 10.0 mg/L. The test media were prepared in natural
river water. Duration of the test was 48 hours. Twenty test organisms
were exposed to each concentration level (4 replicates each with test
organisms) and a control. Water quality parameters pH-value, temperature
and oxygen-saturation, measured after 0, 24 and 48 h, were determined to
be within the acceptable limits.
OLEIC_DimerFA_TETA_PAA has low water solubility and may sorb to organic
and inorganic materials by different mechanisms. Due to these properties
the test item was difficult to test in artificial water (e.g. sorption
to the test organism and walls of the test vessel). Natural river water
contains particulate as well as dissolved organic carbon to which the
test item can sorb partially preventing that the test item settles onto
surfaces. The sorbed fraction of the test item was difficult to extract
from the test system which normally leads to low analytical recoveries.
Nevertheless the test item was present in the test system and therefore
available for exposure (dissolved in water and sorbed).
No determinations of the test item concentrations were carried out.
Preliminary investigations including the use of different analytical
techniques have shown that no specific method was available that met the
required sensitivity in the range of the test concentrations.
All effect levels are given based on nominal concentrations
The 48 h EC50 of OLEIC_DimerFA_TETA_PAA to Daphnia magna was 5.18
mg/L (95% confidence limits: 2.64 - 9.86 mg/L) and the corresponding
NOEC was 2.50 mg/L.
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