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EC number: 701-120-2
CAS number: -
OECD 203, GLP and Klimisch 1 Zebrafish 96 hour LC50 7.07 mg/L
The acute toxicity of the test item OLEIC_DimerFA_TETA_PAA
(batch no.: BB 100860
fish (zebrafish) was determined according to OECD-Guideline for Testing
of Chemicals No. 203 (1992) at Dr.U.Noack-Laboratorien,
31157 Sarstedt, Germany. The study was conducted in natural river water.
A semi-static test, with a test
medium renewal after 48 hours, with the nominal concentrations of0.625
- 1.25 - 2.50 - 5.00 - 10.0 mg/L was performed.
The test media were prepared in natural river water. Duration of the
test was 96 hours. 7 test organisms were exposed to each concentration
level and the control. Water quality parameters pH-value, temperature
and oxygen-saturation, measured after 0, 24, 48, 72 and 96 h, were
determined to be within the acceptable limits.
OLEIC_DimerFA_TETA_PAA has a low water solubility and may sorb to
organic and inorganic materials by different mechanisms. Due to these
properties the test item was difficult to test in artificial water (e.g.
sorption to the test organism and walls of the test vessel). Natural
river water contains particulate as well as dissolved organic carbon to
which the test item can sorb partially preventing that the test item
settles onto surfaces. The sorbed fraction of the test item was
difficult to extract from the test system which normally leads to low
analytical recoveries. Nevertheless the test item was present in the
test system and therefore available for exposure (dissolved in water and
No determinations of the test item concentrations were carried out.
Preliminary investigations including the use of different analytical
techniques have shown that no specific method was available that met the
required sensitivity in the range of the test concentrations.
All effect levels are given based on nominal concentrations
found to be toxic to zebrafish after 96 h at concentrations of 5.00 mg/L
and higher. The LC50after 96 h was 7.07 mg/L and the
corresponding NOEC was 5.00 mg/L.
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