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EC number: 270-648-9
CAS number: 68475-50-3
Aim of this study was to assess the short term toxicity ofTris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium; tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminiumto aquatic invertebrates daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs.
The stock solution 160 mg/L was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changable sentence according to the chemical).-10,20,40,80,160 mg/Lnominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.
The median effective concentration (EC50) for the test substanceTris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium; tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminium, in Daphnia magna was determined to be 322.5 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.
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