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Short-term toxicity to fish:

Short term toxicity to fish was assessed for the target compound Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium(CAS no. 68475-50-3) and its related read- across by reviewing a number of studies. The summary of the results are presented below:

 

Short term aquatic toxic effects of the test compound Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium(CAS no 68475-50-3) was predicted (SSS QSAR, 2016). The study was based on the effects of the test compound on the fish species Danio rerio in 96 hrs duration in a fresh water static system. The predicted data suggests the lethal concentration (LC50) for Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium on the basis of mortality to be 317.83001709 mg/L. This value concluded that the test compound does not exhibit short term toxicity to fish Danio rerio and is non hazardous to the aquatic environment.

Predicted model ECOSAR v1.00 indicate the the 96 hours LC50 was estimated to be 7988.025 mg/l on fish for substance Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium with mortality effects.

In Experimental data from UERL laboratory report 2016 for read across disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate(Cas no. 3567-66-6) 96 h study was conducted on Zebra Fish i.e Danio rerio, at a test concentrations of 100 mg/L. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. 

The lethal concentrations LC50 was found to be >100mg/L.

Also UERL laboratory report 2016 for read across 3-[(2, 4-dimethyl-5-sulphonatophenyl) azo]-4-hydroxynaphthalene-1-sulphonate (Cas no. 4548-53-2) indicate that the 96 h study was conducted on Zebra Fish Danio rerio, at a test concentrations of 100 mg/L. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. 

Whereas for same read across peer reviewed study from The Journal of Toxicological Science, Vol . 3 205-214, 1978 indicate that the Short term toxicity test was performed on Himegaka(Oryzias latipes)was used and acclimated for 10 days in tap water before experiment. In lab, 1 L solution of pH 7 containing 3,000 mg/litre of a Ponceau SX to be tested. 10 tested fish were kept in the tank without direct sunlight for 48 hrs and survival rate was determined.

 From the experiment, TLm (LC 50) value for the short term toxicity to fish was found to be greater than 3000mg/kg.

Whereas experimental study (Chemophere. Vol. 28, No. 12, pp. 2203.2236, 1994) for read across substance 6-aminonaphthalene-1,3-disulphonic acid (118-33-2)indicate that the lethal concentration (LC0) of test substance for fish Leuciscus idus, Bruchydunio rerio,Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulate was found to be 1000 mg/l for 96 hrs.

And other database (Data bank of Environmental Properties of Chemicals (EnviChem), 2014) for read across Sodium naphthionate (Cas no.130-13-2)indicate that the median lethal concentration (LC50) of test substance substance Sodium naphthionate for fish Oryzias latipes was found to be >4000 mg/l for 48 hrs. on the basis of mortality effects.

For read across 2-aminonaphthalene-1-sulphonic acid (Cas no. 81-16-3) from Data bank of Environmental Properties of Chemicals (EnviChem), 2014 indicate the lethal concentration (LC50) for test substance 2 Naphthylamine sulfonic acid on fish species Oryzias latipes was found to be 610 mg/l.

 

Based on above available studies for target chemical and its read across it is concluded that the substance Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium(CAS no. 68475-50-3) not qualify for the aquatic classification as per the CLP criteria.

 

Short-term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity ofTris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium; tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminiumto aquatic invertebrates daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs.

 

The stock solution 160 mg/L was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changable sentence according to the chemical).-10,20,40,80,160 mg/Lnominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The 4% inbition for the test substance,Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium; tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminium in Daphnia magna was observed at 100 mg/L on the basis of mobility inhibition effects in a 48 hour study.Based on the value, indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

Based on above available studies for target chemical it is concluded that the substance Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium(CAS no. 68475-50-3) not qualify for the aquatic classification as per the CLP criteria.

Toxicity to aquatic algae and cyanobacteria

Aim of this study was to assess the short term toxicity ofTris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium; tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminiumto aquatic invertebrates daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs.

 

The stock solution 160 mg/L was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changable sentence according to the chemical).-10,20,40,80,160 mg/Lnominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The median effective concentration (EC50) for the test substanceTris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium; tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminium, in Daphnia magna was determined to be 322.5 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.

 

Toxicity to microorganisms:

Toxicity to micro organism was assessed for the target compound Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium (CAS no 68475-50-3) and its related read- across. The summary of the results are presented below:

Toxic effects of the test compound Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium (CAS no 68475-50-3) was predicted (SSS QSAR, 2016). The study was based on the effects of the test compound on the micro organism species Tetrahymena pyriformis in 48 hrs duration in a fresh water static system. The predicted data suggests the Inhibition growth concentration (IGC50) on the basis of growth inhibition to be 122.513618469 mg/L. This value concluded that the test compound Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium does not exhibit toxicity to micro organism (Tetrahymena pyriformis) and is non hazardous to the aquatic environment.

Whereas experimental study (Chemophere. Vol. 28, No. 12, pp. 2203.2236, 1994) for read across substance 2-aminonaphthalene-1-sulphonic acid (Cas no. 81-16-3)indicate that the effect concentrations i.e EC50 of test substance for bacteria (unadapted biological waste water treatment plant) was found to be >10000 mg/l.

Same experimental source i.e Chemophere. Vol. 28, No. 12, pp. 2203.2236, 1994 also report the the study for another read across 6-amino 1,3-naphthalene-disulfonic acid (Cas no. 118-33-2)which indicate that the effect concentration (EC50) of test substance 6 amino-1,3 naphthalene –disulfonic acid for bacteria (unadapted biological waste water treatment plant) was found to be 1000 mg/l for 24 hrs. on the basis of inhibition effect.

Based on above available studies for target chemical and its relevant read across it is concluded that the substance Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium (CAS no 68475-50-3) non toxic to micro organisms.