Registration Dossier
Registration Dossier
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EC number: 613-701-7 | CAS number: 6485-45-6
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP guideline study according to the German National Standard (DIN).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- no
Test material
- Reference substance name:
- Automatically generated during migration to IUCLID 6, no data available
- IUPAC Name:
- Automatically generated during migration to IUCLID 6, no data available
- Details on test material:
- - Name of test material (as cited in study report): 2,6 Dimethylmorpholin (cis/trans isomere mixture)
- Analytical purity: 98.6% (75.3% cis, 23.3% trans)
- Impurities (identity and concentrations): 1.2% water; 0.2% unknown impurities
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Strain: DSM 50026
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 17 h
Test conditions
- Test temperature:
- 24 ± 1 °C
- pH:
- 4.9 - 9.4
- Nominal and measured concentrations:
- Nominal: 156.25, 312.5, 625, 1250, 2500, 5000, 7500 and 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 300 ml glass Erlenmeyer vessel
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 mL of a Pseudomonas suspension adjusted to a turbidity of 10 TE/F
EFFECT PARAMETERS MEASURED: Photometric determination of light extinction after 17 hours of exposure
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK medium according to DIN 38412, part 8 (draft)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 17 h
- Dose descriptor:
- EC10
- Effect conc.:
- 730 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 17 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 17 h
- Dose descriptor:
- other: EC90
- Effect conc.:
- 1 560 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
