Reaction mass of (2S,6S)-2,6-Dimethylmorpholine and (2R,6R)-2,6-Dimethylmorpholine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure according to guideline with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,6-Dimethylmorpholin

IUPAC Name:

2,6-Dimethylmorpholin

Details on test material:

- Name of test material (as cited in study report): 2,6-Dimethylmorpholin
- Analytical purity: not reported

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Inoculum from BASF STP
- Initial cell/biomass concentration: 1000 mg/L dry weight

Duration of test (contact time):

33 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

400 mg/L

Based on:

DOC

Initial test substance concentration 2

Initial conc.:

666 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Solubilising agent (type and concentration if used): none used
- Test temperature: room temperature
- pH: 6.1 -8.2
- pH adjusted: no


TEST SYSTEM
- Culturing apparatus: all glass bottles (volume: 5L)
- Number of culture flasks/concentration: 1
- Measuring equipment: no data available
- Test performed in open system: no

SAMPLING
- Sampling frequency: after 3hours, 1, 2, 5, 7, 9, 12, 14, 16, 19, 21, 23, 26, 29, 33 days

CONTROL AND BLANK SYSTEM
- Inoculum blank: no. However, a statistical mean value of 17 mg/l was used. This was obtained at the time the test was performed.
- Abiotic sterile control: no
- Toxicity control: no

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

< 10

Sampling time:

33 d

Any other information on results incl. tables

	Time [d]
	3 h	1	2	5	7	9	12	14	16	19	21	23	26	29	33
Theoretical TOC [mg/l]	400	393	386	380	373	367	361	355	349	343	337	331	325	319	314
Measured TOC [mg/l]	392	390	386	391	362	362	360	359	359	347	348	331	333	319	336
Elimination [%]	6	5	4	2	8	6	5	4	2	4	4	5	3	5	- 2
pH	7.5					8.2		7.9		6.9	7.3	7.4	7.2	6.7	6.1

Statistical TOC blank value = 17 mg/L

Comment on the calculation of the elimination (%): samples were taken at regular intervals and the sample volume was always replaced with tap water. Thus, the starting liquid volume remained constant whereas the TOC-reference point was reduced according to the sample volume.

Applicant's summary and conclusion

Interpretation of results:

under test conditions no biodegradation observed