Reaction mass of (2S,6S)-2,6-Dimethylmorpholine and (2R,6R)-2,6-Dimethylmorpholine

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study according to German National Standard (DIN).

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Reference 2

Reference Type:

other company data

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412 part 9

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,6-dimethylmorpholine

EC Number:

205-509-3

EC Name:

2,6-dimethylmorpholine

Cas Number:

141-91-3

IUPAC Name:

2,6-dimethylmorpholine

Details on test material:

- Name of test material (as cited in study report): 2,6 Dimethylmorpholin (cis/trans isomere mixture)
- Analytical purity: 98.6% (75.3% cis, 23.3% trans)
- Impurities (identity and concentrations): 1.2% water; 0.2% unknown impurities

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: SAG 86.81
- Method of cultivation: in OECD medium

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

20 °C

pH:

8.1 - 10.2

Nominal and measured concentrations:

Nominal: 0.98, 1.95, 3.91, 7.81, 15.63, 31.25, 62.5, 125, 250 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, fill volume: Erlenmeyer glass flasks, 250 ml
- Aeration: none
- Initial cells density: 10000 cells/ml
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5

GROWTH MEDIUM
- Standard medium used: yes(OECD medium)

EFFECT PARAMETERS MEASURED: measured after 0 ,24 ,48, 72, 96 hours
- Determination of cell concentrations: fluorimeter

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

218.945 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: recalculated value; 95% CL: 193.568 - 256.771 mg/L

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

79.333 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: recalculated value; 95% CL: 54.691 - 98.471 mg/L