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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given (comparable to guidelines/standards)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986

Materials and methods

Principles of method if other than guideline:
NEN 6501: Determination of acute toxicity with Daphnia magna (1980) with slight modifications (Van Leeuwen et al. 1985).
48 h-LC50 value and the 95 % confidence interval were calculated according to Litchfield JT and Wilcoxon F (1949).
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
4-chloroaniline
EC Number:
203-401-0
EC Name:
4-chloroaniline
Cas Number:
106-47-8
Molecular formula:
C6H6ClN
IUPAC Name:
4-chloroaniline
Details on test material:
- Name of test material (as cited in study report): 4-Cl
- Analytical purity: > 99 %
- Molecular weight: 127.57

Sampling and analysis

Analytical monitoring:
not specified

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
For each concentration, 10 daphnids were used, one animal per jar containing 50 ml medium.
The daphnids were fed on 1x10E+8 cells/L unicellular green algae (C. pyrenidosa).

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.05 mg/L
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL; (0.04-0.06)

Applicant's summary and conclusion

Executive summary:

A test on the acute toxicity of 4 -chloroaniline to the invertebrate Daphnia magna was performed according to

the Dutch Standardization Organization Method NEN 6501. For a test period of 48 hours an LC50 value

of 0.05 mg/l was reported (Maas-Diepeveen and van Leeuwen, 1986).