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EC number: 219-372-2
CAS number: 2425-85-6
main test showed no adverse effects on the algae up to the highest
test concentration (solubility in medium)
The influence of the test item C.I. Pigment Red 3 on the growth of the
freshwater green algal species Pseudokirchneriella subcapitata was
investigated in a 72‑hour static test according to the OECD Guideline
201 (2006) and the Commission Regulation (EC) No 761/2009, C.3.
Due to the low water solubility of the
test item, a dispersion of the test item with the loading rate of
100 mg/L was continuously stirred at room temperature in the dark over
24 hours. Then, the dispersion was filtered. Theundiluted
filtrate with the loading rate of 100 mg/L and dilutions 1:2.2, 1:4.6,
1:10 and 1:22 of the undiluted filtratewere
used as test media. Additionally, a control was tested in parallel.
The preparation of the test media was
based on the OECD Guidance Document on Aquatic Toxicity Testing of
Difficult Substances and Mixtures, 2000.
start and end of the test, the measured test item concentrations in the
test media with the loading rate of 100 mg/L(undiluted
filtrate) were 6.91 and 5.19 µg/L, respectively.
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