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EC number: 219-372-2
CAS number: 2425-85-6
Short-term toxicity to fish
The acute toxicity of the
C.I. Pigment Red 3 to fish (Oryzias latipes) was determined according to
OECD-Guideline for Testing of Chemicals No. 203 (1998). A static limit
test with a saturated solution was conducted.Compared to the control, no
signs of toxicity could be observed in the saturated solution (400mg/L).
Therefore, the NOEC (48h) was determined to be higher than the
concentration of a saturated solution of C.I Pigment Red 3 (>= 400 mg/l
based on the nominal concentration).
Short-term toxicity to
In an acute immobilisation
screening test with Daphnia magna the effect of the saturated solution
of both C.I. Pigment Red 3 and Pigment Orange 5 were determined
according to OECD 202 and Directive 92/69/EC Method C.2. The limit tests
were conducted under static conditions over 48 h.Compared
to the control, no signs of toxicity could be observed in Daphnia magna
with the saturated solution. Therefore, the NOEC (48h) was determined to
be higher than the concentration of a saturated solution of both C.I.
Pigment Red 3 and C.I. Pigment5.
Toxicity to aquatic algae
The toxicity of the test
item (C.I. Pigment Red 3) to the unicellular freshwater green algal
species Pseudokirchneriella subcapitata was determined according to the
principles of OECD-Guideline 201. No inhibiting effects on specific
growth rate and yield were found in the saturated solution of 6mg/L.
The EC50-values for specific growth rate inhibition (ErC50) and
inhibition of yield (EyC50) after 72 h were higher than the saturation
level of the test item.
Toxicity to microorganisms
A Respiration Inhibition
Test with activated sludge according to OECD Guideline No. 209 was
carried out as a limit test for both C.I. Pigment Red 3 and C.I.
Pigment5. The test was performed under static conditions with the
nominal limit concentration of 1000 mg/L. The test item is not toxic at
concentrations ≤ 1000 mg/L to activated sludge of a municipal sewage
Long-term toxicity to
The test item C.I. Pigment Red 3 had no
toxic effects on survival and reproduction of Daphnia magnaafter
the exposure period of 21 days up to the highest test concentration of
mean measured 35 μg/L (nominal 45 μg/L). This test concentration is
clearly above the water solubility of the test item. The 21-day NOEC of
the test item was determined to be at least 35 μg/L.
Thus, the test item C.I. Pigment Red 3
had no toxic effects on Daphnia magna at least up to its
solubility limit in test water.
Long-term toxicity to soil
Mortality and reproduction was not
affected in Earthworm Reproduction Test (OECD Guideline 222) with C.I.
Pigment Red 3. NOEC was at least > 1000 mg/kg soil.
Toxicity to Terrestrial Plants
Seedling emergence and seedling growth
in an OECD 208 study with C.I. Pigment Red 3 for 10 different plant
species did not reveal any toxicity and NOEC was > 1000mg/kg soil.
and chronic tests showed no effects and the substance haslow potential
forbioaccumulation. Therefore the information is conclusive but not
sufficient for classification.
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