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EC number: 931-125-7
CAS number: -
pH value of the control and test solutions was determined to be 8.2
(control), 8.2, 9.1, 9.6, 9.9, 10.2 and 10.5 (highest concentration).
The measured pH after 24 h was reported as 8.0 (control), 8.0, 8.6, 9.0,
9.4, 9.6 and 9.8 (highest concentration).
Observations on the test solution: At the start of the test, all
suspensions were slightly turbid, turbidity increased with dosed amount.
After 24 h all aqueous phases were clear, but undissolving material was
sticking on the bottom of the test vessels.
The EC50 for mobility after 24 h was calculated as 2808 mg/L, EC100 5600
mg/L and NOEC 1800 mg/L. The NOEC for condition of the daphnids
(swimming behavior: slower movements, irregular movements) was
determined as 1000 mg/L.
During the study, a series of previous or further experiments were
- Pre-test with nominal concentrations between 56 and 1000 mg/L (spaced
by a factor of 1.8) plus 10000 mg/L. Only the highest treatment showed
an effect of the test substance on mobility and swimming behavior of
- Unfiltered versus filtered test suspensions in a limit test (1000 and
10000 mg/L): This test was carried out because it was shown in the first
test that the daphnids were physically hampered by undissolved material.
Unfiltered test suspensions resulted in increased immobilization and
affected swimming behavior of daphnids compared to filtered suspensions
(for the highest concentration of 10000 mg/L).
- Main test (results see above)
- ph test: dilution water adjusted to various high pH values because it
was likely that the mobility of daphnids in the tests with Wessalith P
was affected by the rather high pH values of the test suspensions. After
24 h, pH values of 10.5 and 11.0 and after 48 h, additionally pH 10.0
resulted in an increased immobility. Besides, swimming behavior was
affected in the respective treatments, and animals were paler than
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