Registration Dossier
Registration Dossier
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EC number: 217-682-2 | CAS number: 1929-82-4
Toxicity to terrestrial arthropods
Administrative data
Link to relevant study record(s)
Description of key information
LD50 > 100 µg a.s./bee; NOED = 100 µg a.s./bee (oral and contact) Honeybee, Deslandes (2013)
Key value for chemical safety assessment
Additional information
Short term
The toxicity of the test material to terrestrial arthropods was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guidelines OECD 213, OECD 214 and EPA OPPTS 850.3020. The study was assigned a reliability score of 1 in line with the criteria of Klimisch et al. (1997).
Oral and contact tests were carried out to evaluate potential adverse effects of test material on the survival of honeybees, under laboratory conditions, following one application at the limit dose of 100 µg a.s./bee.
A reference item (400 g/L dimethoate) was applied at 3 doses in each test to demonstrate the relative susceptibility of the test organisms and the sensitivity of the test system: the oral test included doses between 0.10 - 0.35 μg a.s./bee and the contact test included doses between 0.10 - 0.30 μg a.s./bee.
Untreated control (oral test), water control (contact test) and acetone treated controls (oral and contact tests) were included to assess the natural mortality of the test organisms and for comparison with the test material treatments.
In the oral test, there was no significant difference between mean mortality in the acetone control and the test material group, after 48 hours (Student-T test, 95 % confidence level). The NOED was found to be 100 µg a.s./bee and both LOED and LD50 were estimated to be > 100 µg a.s./bee.
In the contact test, there was no significant difference between mean mortality in the acetone control and the test material group, after 48 hours (Student-T test, 95 % confidence level). The NOED was found to be 100 µg a.s./bee and both LOED and LD50 were estimated to be > 100 µg a.s./bee.
Long term
In accordance with Column 2 of REACH, Annex X, information requirement 9.4.4, long term toxicity testing on terrestrial invertebrates is not required since the chemical safety assessment does not indicate the need to investigate further the effects on terrestrial organisms to the substance and/or degradation products. The available data are sufficient for classification and labelling purposes, and PBT assessment. Therefore, no further testing is proposed.
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