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EC number: 217-682-2 | CAS number: 1929-82-4
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Not readily biodegradable (4.3% degradation by day 29), OECD 301 B, EPA OPPTS 853.3100, Tunink (2012)
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The ready biodegradability of the test material was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guidelines OECD 301 B and EPA OPPTS 853.3100. The study was therefore assigned a reliability score of 1 in line with the criteria of Klimisch et al. (1997).
During the study duplicate control systems, containing the microbial inoculum with no test or reference substance, were used to determine the endogenous microbial CO2 evolution. Duplicate inoculated test material systems, which were dosed with the test material at a nominal concentration of 20.0 mg C/L, were used to monitor biodegradation of the test material. A reference substance system containing readily biodegradable sodium benzoate at a nominal concentration of 20.0 mg C/L was tested to verify the viability of the microbial inoculum. A toxicity control system containing readily biodegradable sodium benzoate at a nominal concentration of 20.0 mg C/L and the test material at a nominal concentration of 20.0 mg C/ L was tested to monitor potential inhibition by the test material. All systems were sampled for CO2 trapped in 0.2 N KOH on Days 0, 3, 5, 7, 11, 14, 19, 24, 28 and 29. The 0.2 N KOH trapping solutions were analysed for CO2 by inorganic carbon analysis on a total organic carbon analyser for each sample day. The CO2 evolved from the control system was subtracted from the CO2 evolved in the test and reference substance systems.
The test material systems yielded a mean CO2 value of 4.3% by Day 29 of the study. Since the biodegradation value was less than 60% theoretical (ThCO2) by Day 29, the test material cannot be classified as readily biodegradable from the results of this study. Biodegradation in the reference substance system reached 62.7% ThCO2 by Day 11 and 76.7% ThCO2 by Day 29, verifying that the microbial inoculum was viable and active. Biodegradation in the toxicity control system reached 43.3% ThCO2 by Day 29. Since the biodegradation value was greater than 25% ThCO2, the test material can be assumed to not be inhibitory.
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