Hexanedioic acid, mixed esters with decanoic acid, heptanoic acid, octanoic acid and pentaerythritol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Remarks:

Chemical analyses were based on limited number of measured samples and analytical limit of GC detection and quantitation.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test organisms (species):

Daphnia sp.

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

200 mg/L (CaCO3)

Test temperature:

19.8-20.0C

pH:

8.22-8.48

Dissolved oxygen:

60% of saturation (7.1-7.9 mg O2/L)

Nominal and measured concentrations:

0, 324, 1296 and 5076 mg/L (nominal concentration)
WAF is the maximum soluble concentration of the nominal test concentrations after 20 hours of stirring and 4 hours of equilibrating. Only the water phase was used in the definitive test solutions.

Details on test conditions:

- Photoperiod: 16 hr light / 8 hr dark cycle

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 5 076 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: WAF is the maximum soluble concentration of the nominal test concentrations after 20 hours of stirring and 4 hours of equilibrating. Only the water phase was used in the definitive test solutions.

Details on results:

- Mortality of control: No significant immobilization or adverse symptom was observed in the daphnids at any of the tested WAFs compared with controls.

GC-FID analysis for WAF solutions gave limited results due to the limit of GC detection (ca. 10 mg/L). However, several samples showed measurable concentrations (11-13 mg/L) slightly above the GC limit of detection. The test material has limited water solubility (calculated to be 8.16 x 10-8 mg/L, using EpiWin).

Reported statistics and error estimates:

Binomial probability analysis (Stephan et al., 1978): Fisher's exact test

WAF Solution Conc. Nominal load rate (mg/L)	Immobility % (48-hr)
0 Control (untreated)	5%
324	0
648	5
1296	0
2592	0
5076	0

Validity criteria fulfilled:

yes

Conclusions:

48-hr EC50 was > 5076 mg/L WAF (nominal loading rate). No significant immobilization or adverse symptom was observed in the daphnids at any of the tested WAFs compared with controls. Test material could be detectable in the some of the WAF solutions but was close to the limit of analytical detection. The test material has limited water solubility and would be expected to be close to water-saturated levels (WSL) in the tested solutions. The data suggest that test substance would not be expected to cause immobilization at or close to its water saturation levels or water solubility limits (WSL).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

5 000 mg/L