Hexanedioic acid, mixed esters with decanoic acid, heptanoic acid, octanoic acid and pentaerythritol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Remarks:

No chemical analyses were performed to determine the concentration of test substance in water solutions.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

No chemical analyses were performed to determine the concentration of test substance in water solutions.

GLP compliance:

yes

Analytical monitoring:

no

Remarks:

No analysis of water samples were performed

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Loading: 0.26-0.33 g/L.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): The test substance (oil) was maintained as oil in water dispersion/suspension by a propeller (protected against the fish) above the system which created a vortex on the water surface.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Length at study initiation (length definition, mean, range and SD): mean length 25-26 mm

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

203 mg/L CaCO3

Test temperature:

12.0-13.0°C;

pH:

7.83-7.90

Dissolved oxygen:

8.5 to 8.8 mg/L

Nominal and measured concentrations:

Concentrations (nominal): 0 (untreated controls), 99, 493, 1015, 2001 and 5017 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 40 L glass vessels containing 30 L well water
- No. of organisms per vessel: 20 / treatment

OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h dark cycle

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 5 017 mg/L

Nominal / measured:

nominal

Conc. based on:

other: oil in water suspension/dispersion

Basis for effect:

mortality (fish)

Remarks on result:

other: see Details on results

Details on results:

- Mortality of control: No mortality was observed in the fish at any of the nominal concentrations which ranged from 99 mg/L to 5017 mg/L

Remarks: 1) The fish were relatively small (25-26 mm) compared to that recommended by EC L 383 A: (6020 mm). Since small fish may be more sensitive, this may be acceptable in a worst case approach.
2) Because the test substance is not soluble in water, it is kept in suspension by a propeller situated above the water surface, utilizing oil in water dispersion method.
3) The LC50 is determined using the nominal concentration, since test material was water-insoluble.
4) The temperature during the study was at the lower range of temperature recommended (12-13°C versus EC L 383 A recommended 12-17°C).

Sublethal observations / clinical signs:

Nominal test conc. - Loading Level (mg/L)	Mortality (96-hr)
0 Control (untreated)	0
99	0
493	0
1015	0
2001	0
5017	5

No mortality was observed in the fish at any of the nominal concentrations which ranged from 99 mg/L to 5017 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

The 96-h LC50 was > 5017 mg/L (nominal concentration, oil in water suspension/dispersion).
Analyses to determine actual concentrations of the test material were not performed. The nominal test concentrations in exposure samples were all above the water solubility of the test material (calculated to be 8.16 x 10-8 mg/L, using EpiWin). Hence, the ecotoxicity data indicate that the test material would not be expected to cause acute toxicity in fish at its water saturation limit or water solubility limit (WSL).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

5 000 mg/L