Registration Dossier
Registration Dossier
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Reaction mass of 5,5'-{(phenylmethanediyl)bis[benzene-4,1-diyl-diazene-2,1-diyl]}bis{1-[3-(dimethylamino)propyl]-4-methyl-6-oxo-3-(pyridinium-1-yl)-1,6-dihydropyridin-2-olate} hydrochloride and 5,5’-[3,4’-(phenylmethanediyl)diphenylene]bis(diazene-2,1-diyl)bis{1-[3-(dimethylamino)propyl]-4-methyl-6-oxo-3-(pyridinium-1-yl)-1,6-dihydropyridin-2-olate} hydrochloride
EC number: 700-312-3 | CAS number: -
Endpoint summary
Administrative data
Description of key information
Additional information
Acute aquatic toxicity
Valid studies have been conducted with the analogue substance. The applicability of the read across approach had been confirmed by the registrant via an expert statement (see IUCLID chapter 13).
Summary of results of ecotoxicity endpoints for the substance registered, derived from short term aquatic toxicity studies on the analogue substance:
LC50 fish: 1.66 mg/L
EC50 Daphnia: 7.2 mg/L
EC50 algae (growth): 1.76 mg/L
With all acute aquatic effect values (LC50 and EC50) > 1 mg/L, no classification of the substance registered for acute aquatic toxicity under CLP and DSD regulation.
Chronic aquatic toxicity
CLP classification:
With the 2nd amendment of the CLP regulation, results from aquatic long-term studies are to be preferred for classification for chronic aquatic toxicity.
At this point in time only the results from the algae toxicity study (which is a multi-generation study) can be used directly for chronic aquatic toxicity classification. With a NOEC of 0.48 mg/L for non-rapidly degradable substances the resulting classification is "Aquatic Chronic Category 2", H411 "Toxic to aquatic life with long lasting effects".
Above stated classification also is being confirmed when using the results of the acute fish toxicity study (LC50 = 1.66 mg/L, i.e. the most sensitive acute study) in combination with the fact that substance registered is not rapidly degradable.
DSD classification:
The most sensitive acute aquatic toxicity study was the study with fish, revealing an LC50 of 1.66 mg/L. In combination with the fact that substance registered is not rapidly degradable, the resulting classification is "Dangerous for the environment" N; R51/53, "Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment". The DSD classification fits to above stated CLP classification.
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