(undec-10-enoylamino)acetic acid

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

Principles of method if other than guideline:

This study is a pre-test

GLP compliance:

not specified

Remarks:

The test was conducted according to OECD 201 but the Good Laboratory Practice certificate is not included in the test report.

Analytical monitoring:

yes

Details on sampling:

The test item was stored in a closed vessel at room temperature.
A stock solution of an appropriate concentration in deionised water was prepared. The
stock solution then was used to prepare solutions of the concentrations to be tested.

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Desmodesmus subspicatus CHODAT, unicellular freshwater green alga. The culture was
obtained in Feb. 2009 by the „Collection of Alga“, Institute of Plant Physiology of the University
of Göttingen. The alga are kept as stock culture on solid agar at 8 oC. Three or four
days before the start of the test, an aliquot containing a few cells is brought into algal medium
and incubated. The resulting culture is growing exponentially.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test temperature:

23 to 26°C

pH:

7.1 to 8.1

Nominal and measured concentrations:

nominal: 0 / 0.1 / 1 / 10 / 100 mg/L

Reference substance (positive control):

no

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

>= 10 - <= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC100

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Validity criteria fulfilled:

yes

Conclusions:

NOEC 72 h = 1 mg/L
72h ErC50= 10 – 100 mg/L
72h EbC50= 10 – 100 mg/L
72h EyC50= 10 – 100 mg/L
72h EC100= 100 mg/L

Executive summary:

Pre-test: Study Report following OECD 201 / EN ISO 8692 / EU-Guideline C.3: Toxicity of Lipacide UG against Desmodesmus subspicatus

The aim of this study was to determine the possibility to do an OECD 201 test.

RESULTS

NOEC 72 h = 1 mg/L

72h ErC50= 10 – 100 mg/L

72h EbC50= 10 – 100 mg/L

72h EyC50= 10 – 100 mg/L

72h EC100= 100 mg/L

ANALYTICAL MEASUREMENT OF STABILITY

The stability of the test item in test medium was measured.

A solution of 40 mg/L was measured at t = 0h and 72h. The area at t = 0h was 11 and at t = 72h 6.9, resulting in a recovery of 63%. Therefore it can be stated that the test item is not stable in the test medium for the algal test.

As the test item is not stable and it is not technically possible to use a semi-static protocol for algae, no main study was performed.

Description of key information

A pre-test study report following OECD 201 / EN ISO 8692 / EU-Guideline C.3 : Toxicity of the test substance against Desmodesmus subspicatus was done to determine the possibility to do an OECD 201 test.The test item is not stable and it is not technically possible to use a semi-static protocol for algae, no main study was performed.

Key value for chemical safety assessment

EC50 for freshwater algae:

10 mg/L

EC10 or NOEC for freshwater algae:

1 mg/L

Additional information

ErC50 (72h) = 10 -100 mg/L