(undec-10-enoylamino)acetic acid

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD guideline, GLP study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

1996-20-02

Analytical monitoring:

no

Details on test solutions:

a stock solution (500 mg of the test substance per litre) was prepared 52 minutes before the start of the exposure, dissolving the test substance into dilution water; pH of this stock solution was adjusted to make the test substance soluble in our experimental conditions at the request of the study monitor.

The test substance concentrations in the test media were obtained adding the appropriate volumes of the stock solution in the respective test flasks.
-for each flask, a mixture of dilution water, synthetic sewage (16mL) and inoculum (200mL), was added at 12-minutes intervals, to the substance (expected for the control flasks, without any substance), to form a final volume of 500 mL per flask.
-pH was adjusted in the test media around pH 7.5-8.0 to make the test substance soluble in our experimental condtions at the request of the study monitor.

Test organisms (species):

activated sludge

Details on inoculum:

mixture of different strains of micro-organisms (inoculum) found in activated sludge from a sewage treatment plant treating predominantly domestic sewage (La Fouillous - Loire - FRANCE
The sludge was used on the day of collection
the sludge was mixed during 1 minute in order to destroy the coarse particles
the concentration of suspended solids was evaluated from a small amount of sludge
the sludge was centrifugated and the suspended solids obtained were washed before to be resuspended for the preparation of the inoculum (centrifugated 3 times with deminineralized water containing synthetic sewage for 5 minutes at 2000 rpm, with discarding of the supernatants)
the suspended solids were then re-suspended into synthetic sewage so as to obtain a nominal concentration of suspended solids of approximately 2 grammes per ltre in the inoculum
this inoculum was kept aerated and at thermoregulated temperature (20 +/- 2°C) until its using for the preparation of the media

Test type:

static

Water media type:

freshwater

Total exposure duration:

3 h

Test temperature:

19.1-23.4°C

pH:

from 7.49 to 7.97

Nominal and measured concentrations:

1 / 10 / 100 mg/L

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Results with reference substance (positive control):

ihnibition percentage after 3 hours:
26% at 5 mg/L
74% at 15 mg/L
77% at 30 mg/L

Validity criteria fulfilled:

yes

Conclusions:

EC50-3 hours > 100mg/L

Executive summary:

Inhibition of respiration in activated sludge, in compliance with OECD guideline n°209 and EEc directive 87/302

Method

-micro-organisms from activated sludge (800 mg of suspended solids per litre of medium) were exposed to a range of concentrations of test substance for 3 hours; 1, 10 , 100mg test substance per litre of test medium

-two contro media (without test substance) were tested, one at the beginning of the test, the other one at the end of the test

-the dissolved oxygen concentration was measured in 300 mL samples from each after approximately 3 hours exposure

-a reference substance, 3,5 -dichlorophenol, was tested under the same conditions in order to control the sensitivity of the activated sludge (3 concentrations)

-the criteria of quality for the study, as notified in the OECD and EEC standards and relative to the level of oxygen consumption in the two control media and to the toxicity of 3,5 -dichlorophenol, were respected

Results

Taking into account the results obtained under the experimental conditions employed, these ones may be expressed as follows:

EC50 -3 hours higher than 100 mg/L

Description of key information

Inhibition of respiration in activated sludge, in compliance with OECD guideline n°209 and EEc directive 87/302 test was performed and the (EC₅₀ -3 hours) was found to be higher than 100 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L