Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): NAPHTHALIMID TROCKEN BER. 100%
- Lot/batch No.:P.265
- Analytical purity: 99.6 %

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
activated sludge
Details on inoculum:
The activated sludge was taken from a Iaboratory wastewater plants treating municipal sewage.
- Initial biomass concentration: 1 g/l

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
180 min

Test conditions

Test temperature:
20 ± 2 °C
pH:
6.7 before correction
7.1 after correction
Nominal and measured concentrations:
Nominal test substance concentration: 1002 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium: 8 ml/vessel 1 00-fold concentrated OECD medium

Reference substance (positive control):
yes
Remarks:
3,5-dichlorphenole

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
Change in oxygen consumption rate compared with blank control after 180 min 1002 mg/l --> -6 % (respiration stimulation)

Applicant's summary and conclusion