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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study, no analytical monitoring

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Naphthalene-1,8-dicarboximide
EC Number:
201-379-7
EC Name:
Naphthalene-1,8-dicarboximide
Cas Number:
81-83-4
Molecular formula:
C12H7NO2
IUPAC Name:
3-azatricyclo[7.3.1.0⁵,¹³]trideca-1(13),5,7,9,11-pentaene-2,4-dione
Details on test material:
- Name of test material (as cited in study report): Naphthalimid
- Analytical purity: > 95 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was mixed with the test water and homegenised by Ultra-turrax
- Evidence of undissolved material (e.g. precipitate, surface film, etc): undissolved substance accumulated at the bottom of the test auquaria.

Test organisms

Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 2.5 - 3.5 cm


ACCLIMATION
- Type and amount of food: Tetra Mm, Tetra Werke, Melle, Germany; ad libitum
- Feeding frequency: twice daily

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
22 ± 1 °C
pH:
7.0 - 7.9
Dissolved oxygen:
5.0 - 8.7 mg/l
Nominal and measured concentrations:
Nominal test substance concentrations:
0, 100, 2500, 3500 and 5000 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquaria (30x22x24 cm)
- Fill volume: 10 l
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water according to ISO/DIS 7346/1


OTHER TEST CONDITIONS
- Photoperiod: 12:12 h day-night regime
- Light intensity: ca. 700 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 2 - 4, 24, 48, 72 und 96 hours

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
< 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
behaviour
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
ca. 2 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 3 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
ca. 5 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
At 1000 and 2500 mg/l the symptoms like reduced activity and necrosis of the gills were observed.
Reported statistics and error estimates:
No LC50-calculation

Any other information on results incl. tables

Sublethal observations / clinical signs:
Mortalities of fishes:
--------------------------------------------
¦ conc.[mg/l] ¦ 1000 ¦ 2500 ¦ 3500 ¦ 5000 ¦
============================================
¦ after 48 h ¦ 0/10 ¦ 0/10 ¦ 2/10 ¦ 10/10 ¦
--------------------------------------------
¦ after 96 h ¦ 0/10 ¦ 0/10 ¦ 4/10 ¦ 10/10 ¦
--------------------------------------------

Applicant's summary and conclusion