Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): NAPHTHALIMID TROCKEN BER. 100%
- Lot/batch No.: P.265
- Analytical purity: 99.6 area %

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge from laboratory wastewater plants treating municipal sewage.
- Concentration of sludge: 30 mg/l.
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
27 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
80 - 90
Sampling time:
28 d
Details on results:
RS-Freetext: - TOC: 731 mg/g - adaptation phase: 15 days, degradation phase: 13 days - degradation in the inhibition control(CO2/ThCO2): 50-60% Kinetic of test substance (in %): ca. 3 after 14 day(s) ca. 23 after 17 day(s) ca. 57 after 21 day(s) ca. 68 after 24 day(s) ca. 89 after 28 day(s) Kinetic of control substance (in %): ca. 70 ... 80 after 14 day(s)

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable