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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412 "Testverfahren mit Wasserorganismen (Gruppe L)."
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethyl diphenylphosphinite
EC Number:
211-951-8
EC Name:
Ethyl diphenylphosphinite
Cas Number:
719-80-2
Molecular formula:
C14H15OP
IUPAC Name:
ethyl diphenylphosphinite
Details on test material:
- Name of test substance (as cited in study report): Ethosydiphenylphosphin
- Batch No.: 890011
- Purity: 94.2 %
- Homogeneity: ensured since the test substance is a genuine liquid
- Physical state: liquid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
- Common name: Golden orfe
- Supplier: Fischzucht Paul Eggers, Hohenwestedt, Germany
- Body length: 8.4 cm (7.5 - 9.3 cm)
- Body weight: 6.0 g (4.4 - 7.6 g)
- Housing: Fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air
Photoperiod: 16 h light : 8 h dark
Water temperature: 18 - 22°C
- Water parameters of housing water: - total hardness: about 2.5 mmol/L
- acid capacity: about 5.5 mmol/L
- oxygen content: > 60% of max. saturation
- pH: about 8.0
- Duration of housing and adaptation: 4 months
- Mortality during last 2 weeks of housing: about 0.1%
- Mortality during adaptation period: 0%
- Medical treatment: twice with 0.05 mg/L malachite green chloride
once with 10 mg/L tetracycline hydrochloride
- Diet: growing feed F/B 50, SSNIFF Spezialdiaeten GmbH, Soest, Germany
frequency: ad libitum

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
2.5 mmol/L
Test temperature:
22 +/- 1°C
pH:
7.0 - 7.5
Dissolved oxygen:
6.9 - 8.1 mg/L
Nominal and measured concentrations:
- Test concentrations (nominal): 4.64, 10, 21.5, 46.4 and 100 mg/L
Details on test conditions:
TEST WATER
- Reconstituted freshwater according to DIN 38412, part 11
- Preparation from fully demineralized tap water
- Conductivity: max. 10 micro mho
- Resalting: - 294.0 mg/L CaCl2 * 2 H2O
- 123.3 mg/L MgSO4 * 7 H2O
- 63.0 mg/L NaHCO3
- 5.5 mg/L KCl
- Aeration: continuous with oil-free air
- Test water ready for use:
- Acid capacity: 0.8 mmol/L
- Ratio Ca/Mg-Ions: 4:1
- Ratio Na/K-Ions: 10:1
- pH: about 8.0

TEST SYSTEM
- Test vessel: all-glass aquarium (30 x 22 x 24)
- Volume of water: 10 L
- No. of animals per test concentration: 10
- Loading (g fish / L test water): 6.0

FURTHER CONDITIONS
- Adaptation to test water and test temperature: 3 days
- Withdrawl of food: 1 day before and during exposure

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 21.5 - < 46.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Visual observations: the aquarium contents were increasingly cloudy with increasing concentration

Applicant's summary and conclusion