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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP compliant guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1997

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethyl diphenylphosphinite
EC Number:
211-951-8
EC Name:
Ethyl diphenylphosphinite
Cas Number:
719-80-2
Molecular formula:
C14H15OP
IUPAC Name:
ethyl diphenylphosphinite
Details on test material:
- Name of test substance (as cited in study report): Ethoxidiphenylphosphin dest.
- Molecular weight: 230 g/mol
- Purity: 95.6 %
- Parameters of test substance: TOC: 730 mg/g, ThOC: 730 mg/g, ThCO2: 2679 mg/g

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Inoculum: activated domestic and industrial sludge from laboratory sewage-treatment plant
- The concentration of dry substance was 30 mg/l
Duration of test (contact time):
29 d
Initial test substance concentration
Initial conc.:
28 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
> 20 - < 30
Sampling time:
28 d
Details on results:
- Duration of adaptation phase: 4 days
- Duration of degradation phase: 24 days

Kinetic of test substance (in %):
ca. 12 after 5 day(s)
ca. 14 after 7 day(s)
ca. 18 after 14 day(s)
ca. 20 after 21 day(s)
ca. 22 after 28 day(s)

- Degradation in the inhibition control (CO2/ThCO2): 40-50 % after 14 days

BOD5 / COD results

Results with reference substance:
- Degradation of reference substance (% CO2/ThCO2): 80 - 90 after 14 days
- Kinetic of control substance (in %):
ca. 80 ... 90 after 14 day(s)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: Not readily biodegradable (according to OECD criteria)