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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP compliant guideline study, available as unpublished report, no restrictions, fully adequate for assessment.
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
GLP compliance:
yes
Radiolabelling:
no
Details on sampling:
- Sampling intervals/frequency for test organisms: analyzed once before first analysis of the test fish, and thereafter at the same time as analysis of test fish (single samples)
- Sampling intervals/frequency for test medium samples: fish exposed to both concentrations of test item were analysed five times during the exposure period. Four fish were taken out at each sampling time and divided into two groups, each analyzed individually (because one fish was too small to take out the stored sample for the measurement of lipid content). Control fish were analyzed before the start of the experiment and after experimental completion. Four fish were taken out at each sampling time and divided into two groups, each analyzed individually. In addition, two fish were taken out and three groups (two fish per group) were used for measurement of lipid contents.
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):
test water: 1 mL of the test water from Level 1 was taken from the tank and filled up to 10 mL with water for recovery test, 10 mL of the test water from Level 2 was taken from the tank and left undiluted.
test fish: fish were taken from the test tank, measured for weight and body length, chopped into pieces, and homogenised using polytron for 1 min on ice water, the homogenate was washed with 4 mL tetrahydrofuran and centrifuged at 4000x g for 5 min at -5 ºC. The supernatant was filtrated using absorbent cotton and filled up to 50 mL with tetrahydrofuran (volumetric flask). 20 µL was taken out and filled up to 10 mL with water.
Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
PREPARATION OF SPIKED WATER
- Details of spiking: the test sample and Tween-80 (20 times amount of it) were dissolved in N,N-dimethylformamide to prepare 10.0 g/L stock solution. For Level 1 1000 mg/L of stock solution was added to N,N-dimethylformamide to prepare 50.0 mg/L stock solution. For Level 2 1000 mg/L of stock solution was added to N,N-dimethylformamide to prepare 5.0 mg/L stock solution.
- Controls: Tween-80 was dissolved in N,N-dimethylformamide to prepare 1000 mg/L stock solution as the concentration of Tween-80.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween-80
- Concentration of vehicle in final test solution: 1000 mg/L
Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: carp
- Source: Fukuoka fisheries and marine technology research center, freshwater fisheries laboratory.
- Age at study initiation (mean and range, SD): yearling fish
- Length at study initiation (length definition, mean, range and SD): 6.2 - 10.0 cm
- Feeding during test: yes
- Food type: feed for fry of carp
- Amount and frequency: amount corresponding to about 2% of total body weight was fed twice a day in halves (once a day in all at holiday).

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): yes
- Food type: feed for fry of carp
- Amount and frequency: amount corresponding to about 2% of total body weight was fed twice a day in halves (once a day in all at holiday).
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
60 d
Total depuration duration:
3 d
Hardness:
13.2 mg/L (s Ca and Mg)
Test temperature:
23.8 to 25.0 ºC
pH:
7.2 to 7.8
Dissolved oxygen:
5.7 to 7.6 mg/L
TOC:
1.6 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 70-L glass tank
- Type (delete if not applicable): special tank for volatile items
- Material, size, headspace, fill volume: 70 L
- Renewal rate of test solution (frequency/flow rate): 0.04 mL for stock solution and 2000 mL/min for dilution water, stock solution were renewed 2 - 3 times each month
- No. of organisms per vessel: 54 for Level 1 and 2, 14 for control
- No. of vessels per concentration (replicates): 1
- No. of vessels per control / vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: groundwater from the premises of Kurume Laboratory
- Particulate matter: <1 mg/L
- Pesticides: <0.001 mg/L
Nominal and measured concentrations:
Nominal:
Level 1: 1 μg/L
Level 2: 0.1 μg/L

Measured:
Level 1: 0.952 μg/L
Level 2: 0.0956 μg/L
Reference substance (positive control):
no
Lipid content:
2.74 %
Time point:
start of exposure
Lipid content:
2.62 %
Time point:
end of exposure
Key result
Conc. / dose:
1 µg/L
Temp.:
>= 24 - <= 25 °C
Type:
BCF
Value:
2 600 L/kg
Basis:
whole body w.w.
Calculation basis:
steady state
Key result
Conc. / dose:
0.1 µg/L
Temp.:
>= 23.8 - <= 24.7 °C
Type:
BCF
Value:
2 400 L/kg
Basis:
whole body w.w.
Calculation basis:
steady state
Key result
Elimination:
yes
Parameter:
DT50
Depuration time (DT):
1 d
Details on results:
- Behavioural abnormalities: no abnormality in behaviour or appearance was noted
Validity criteria fulfilled:
yes
Conclusions:
The BCF of AC-6000 is 2600 L/kg in carp (Cyprinus carpio).
Executive summary:

The bioaccumulation of AC-6000 in fish was studied in accordance with OECD guideline 305 (Kurume Laboratory, 2006b).

In this GLP-compliant study yearling of carp (Cyprinus carpio) was exposed to 0.1 and 1 μg/L (nominal concentrations) under flow-through conditions at 25 ºC. The test concentrations were analytically verified and were maintained at more than 91% of nominal concentrations. The steady-state bioconcentration factors (BCF) were 2,400 L/kg at 0.1 μg/L and 2,600 L/kg at 1 μg/L. During the test no abnormality in behaviour or appearance was noted. The BCF of 2,600 L/kg is used in the assessment.

Description of key information

The bioaccumulation of AC-6000 in fish was studied in accordance with OECD guideline 305 (Kurume Laboratory, 2006b).

In this GLP-compliant study yearling of carp (Cyprinus carpio) was exposed to 0.1 and 1 μg/L (nominal concentrations) under flow-through conditions at 25 ºC. The test concentrations were analytically verified and were maintained at more than 91% of nominal concentrations. The steady-state bioconcentration factors (BCF) were 2,400 L/kg at 0.1 μg/L and 2,600 L/kg at 1 μg/L. During the test no abnormality in behaviour or appearance was noted. The BCF of 2,600 L/kg is used in the assessment.

Key value for chemical safety assessment

BCF (aquatic species):
2 600 L/kg ww

Additional information

The bioaccumulation of AC-6000 in fish was studied in accordance with OECD guideline 305 (Kurume Laboratory, 2006b).

In this GLP-compliant study yearling of carp (Cyprinus carpio) was exposed to 0.1 and 1 μg/L (nominal concentrations) under flow-through conditions at 25 ºC. The test concentrations were analytically verified and were maintained at more than 91% of nominal concentrations. The steady-state bioconcentration factors (BCF) were 2,400 L/kg at 0.1 μg/L and 2,600 L/kg at 1 μg/L. During the test no abnormality in behaviour or appearance was noted. The BCF of 2,600 L/kg is used in the assessment.