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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 Dec.2011 - 17 Feb.2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Biodegradation is determined by the EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report Date:
2012

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
Activated sludge from a bilogical sewage treatment plant was used. The chosen plant treats mostly domestic sewage. The sludge was taken from the activation basin of the ESn (Stadtentsorgung Neustad) sewage treatment plant.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
424.88 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Reference substance
Reference substance:
aniline
Remarks:
624.2mg/L in desionised water

Results and discussion

Test performance:
Six samples were taken in order to determinate the DOC (after 3 hours and on days 2, 6, 9, 16 and 28). DOC was determinated as difference between Total Carbon and Inorganic Carbon using a carbon analyser (Analytik Jena).
% Degradationopen allclose all
Parameter:
% degradation (DOC removal)
Value:
-1
Sampling time:
2 d
Parameter:
% degradation (DOC removal)
Value:
-0.5
Sampling time:
6 d
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
9 d
Parameter:
% degradation (DOC removal)
Value:
38.6
Sampling time:
16 d
Parameter:
% degradation (DOC removal)
Value:
92.1
Sampling time:
28 d
Details on results:
Ready degradability is defined in the guideline as degradation surpassing 70% within 10 days after reaching a level of 10%. Therefore, the test item cannot be considered as "readily biodegradable". Degradation surpassed 70% within 28 days, though. Therefore, the test item may be considered as biodegradable within 28 days either. Therefore test item can be considered as "biodegradable".

BOD5 / COD results

Results with reference substance:
Degradation of the positive control was 100% after six days.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: biodegradable
Conclusions:
1-Cyanocyclopropanaminium chloride is biodegradable.