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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Short-term toxicity to aquatic invertebrates was determined according to EU Method C.2 (Acute toxicity for Daphnia Test)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report Date:
2012

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Initial Concentration of the test item : 99.8%
Sample storage conditions before analysis: in a closed vessel dark and dry at room temperature.
On test solutions : concentrations were measured at the beginning and at the end of the test. The correlation between measured concentrations after 48 hours and nominal concentrations was very good (92 to 99%).

Test solutions

Vehicle:
yes
Details on test solutions:
A stock solution containing 100.1mg/L of test item in water was diluted to give a series of concentrations : 10/18/32/56/100mg/L.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
-Variety: STRAUS
-Strain: Berlin
-Source: Umweltbundesamt Berlin
-Age: between 0 and 24 hours
Details:
Daphnia Magnia is bred in LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
2.502 mmol/L
250 mgCaCO3/L
Test temperature:
18.5 - 21.1°C
pH:
Range of 6.4 to 7.6
Dissolved oxygen:
Above 8.7mg/L throughout the test.
Salinity:
5.8 mg/L
Nominal and measured concentrations:
10mg/L: 9.55mg/L at 0h and 8.8mg/L at 48h.
18mg/L: 17.27mg/L at 0h and 16.52mg/L at 48h.
32mg/L: 30.93mg/L at 0h and 30.49mg/L at 48h.
56mg/L: 54.75mg/L at 0h and 54.28mg/L at 48h.
100mg/L: 99.88mg/L at 0h and 97.36mg/L at 48h.
Details on test conditions:
Replicate: four vessels, each containing 20+-5mL test solution and five daphnia.
Control: four vessels, each containing 20+-5mL dilution water and five daphnia.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (CAS No.7778-50-9)

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
56 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
100 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
56 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
75 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
None of the daphnia died or shown any signs of abnormal behaviour throughout the test.
Only the highest concentrated treatment (100mg/L) showed toxicity (100% immobilisation after 48 hours), due to acidic property of the test item and the very low pH value in the test solution at 100mg/L.
Results with reference substance (positive control):
The 24h-EC50 of potassium dichromate was tested in a current reference test. The value was determined as 1.7mg/L, lying within the demanded range of 0.6-1.7mg/L.
Immobilisation in the controls may not exceed 10% and immobilisation in the controls was 10%.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
24h-NOEC = 56 mg/L
48h-NOEC = 56 mg/L
24h-EC50 = 100 mg/L
48h-EC50 = 75 mg/L
This result will not conduct to a classification "dangerous for environment".