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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

As the substance is poorly soluble in water, most of the studies are performed using Water accommodated fractions (WAFs) and all the results are based on nominal loadings. No study could lead to the calculation of an EL50.
72 hour EL50 >100 mg/l
NOELR ≥100 mg/l

Key value for chemical safety assessment

Additional information

Measured toxicity data are available for Shell GTL Solvent GS250 (Hydrocarbons, C14-C16, n-alkanes, isoalkanes, <2% aromatics) to the freshwater green alga Pseudokirchneriella subcapitata (Vryenhoef, 2014). The test was conducted under static (no renewal of the test media) conditions in accordance with OECD Test Guideline 201 and method C3 of EC Regulation No. EC 761/2009. Appropriate modifications to the test and media preparation procedures were made to take account of the test substance containing multiple constituents, having low solubility in water and being potentially volatile. No effects on growth (expressed in terms of yield (y) and growth rate (r)) of P. subcapitata were observed after 72 hours exposure to the test medium prepared as a water-accommodated fraction (WAF) at a loading rate of 100 mg/l; 72-hour EyL50and ErL50values were >100 mg/l and NOELR values were ≥100 mg/l. Total Organic Carbon (TOC) analysis of the 100 mg/l loading rate WAF test preparation at 0 hours showed a measured concentration of 6.14 mg C/l. A decline in measured carbon concentration was observed at 72 hours to 3.04 mg C/l. The results of the test are considered to be reliable.

Fourteen other study reports were available for this end-point, all dealing with the toxicity of the substance “Hydrocarbons, C14-C18,n-alkanes, isoalkanes, cyclics, < 2% aromatics” on algae growth rate. All the studies used the marine species Skeletonema costatum. None of the studies allowed calculating 48h-EL50 that was never reached. All LL50 are superior to 10000 mg/L based on WAF studies.