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EC number: 217-886-1 | CAS number: 1999-85-5
Under semi-static conditions of 72 h-renewal, the effect on the reproductive output of Daphnia magna exposed to the test substance (meta-Diisopropanolbenzene) for 21 days was conducted according to the following guidelines: “The guidelines for the testing of chemicals”, SEPA(HJ/T 153 -2004); Procedure 204 of the Daphnia magna, Reproduction Test”, OECD Guidelines for Testing of Chemicals, 211, adopted on 21 September 1998.etc.
During the whole test period, the pH values of the control mediums and Test Media were between 7.5 and 7.9, and the Dissolved Oxygen (DO) values varied from 90% to 98% of the air saturation at the test temperature, the hardness values varied from 119 to 121 mg(CaCO3)/L, and the temperature of the Test Media was maintained in the range of 20oC to 21oC. All animals in the control were alive and the mean number of live offspring produced per parent animal surviving at the end of the test was > 60. The coefficient of variation around the mean number of living offspring produced per parent animal in the control(s) was ≤25%. So the study met the acceptability criteria. Therefore the test was considered valid.
In order to confirm the stability of the test item in the test medium, concentrations of the test samples during thetest were analyzed by UPLC-PDA. A linear regression equation was obtained with the mean peak squares(A) vs. the concentration of the testsubstance (c, mg/L): A =c-1220.4, with good linearity of r2=0.999. The results show that linearity for the concentration range of 0.10 mg/L to 2.00 mg/L is good. Besides, the minimum detection amount of UPLC-PDA for the test substance is 5.0×10-10g, and the minimum detection concentration for water sample is 0.10mg/L. The analytical results showed that the concentration of the test substance is consistent in the test medium throughout the test period (deviation within 20% of the initial concentration). Thus a semi-static method of 72h-renewal test procedure was reasonable.
The results showed that under valid semi-static test conditions (72 h-renewal), the LOEC is greater than 1.60 mg/L (measured concentration 1.60 mg/L). The NOEC is 1.60 mg/L (measured concentration 1.60 mg/L). According to“The guidelines for the hazard evaluation of new chemical substances (HJ/T 154 -2004)”, belongs to low toxic chemical for Daphnia magna.
The results showed that under valid semi-static test conditions (72 h-renewal), the LOEC is greater than 1.60 mg/L (measured concentration 1.60 mg/L). The NOEC is 1.60 mg/L (measured concentration 1.60 mg/L).
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