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EC number: 217-886-1 | CAS number: 1999-85-5
The inherent biodegradation test of meta-Diisopropanolbenzene was performed according to “The guidelines for the testing of chemicals” (HJ/T 153 -2004), “The guidelines for the testing of chemicals” (SEPA.: Environmental Sciences Press. 2004), and Procedureof the ‘Guidelines for Testing of Chemicals’ of the OECD: “Inherent Biodegradability: Modified MITI Test (II)” (1981)etc.The inherent biodegradability of meta-Diisopropanolbenzene was determined in a 28 -day Biochemical Oxygen Demand (BOD) test and the analysis of residual chemical of meta-Diisopropanolbenzene in BOD bottles in an aerobic, aqueous medium. During the test, the temperature was kept at (25±1)°C. The test was valid because the level of biodegradation of the reference substance sodium benzoate exceeded 40% after 7 days, and 65% after 14 days. Based on the residue analysis, biodegradation of meta-Diisopropanolbenzene was 0% in the “abiotic” vessel during the testing period. The BOD results showed that biodegradation of meta-Diisopropanolbenzene was <1% after 28 days. Therefore, meta-Diisopropanolbenzene has no inherent biodegradability under this test condition.
The study was conducted according to the guidelines of SEPAHJ/T 153-2004, “the guidelines for the testing of chemicals”; and with the reference to OECD Procedure 301D etc. Under valid conditions, the ready biodegradability of meta-Diisopropanolbenzene was determined in a 28-day dissolve oxygen depletion using secondary effluent from a domestic waste water treatment plant. During the test, the temperature was kept at (20±2)°C. The test is valid because the level of biodegradation of the reference substance reached 67.2% within 14 days. Oxygen consumption in inoculum blanks was 1.19mg O2/litre after 28 -day test. The test substance did not inhibit the degradation of the reference substance and was therefore not toxic to the inoculum. Thus the study met the acceptability criteria prescribed by the protocol and was considered valid. Finally the results showed that under valid conditions, the biodegradation rate of meta-Diisopropanolbenzene was 0% biodegradation after 28 -days; while the reference substance (sodium benzoate) reached 67.2% after 14 days, which indicated that meta-Diisopropanolbenzene is not readily biodegradable.
Trap Sampling and Titre Volumes (0.05 M HCl)
Background Titre (mL)
Test Item 1(mL)
Test Item 2(mL)
* Different background titre applied to Trap 3 analysed on Day 5; this was due to Trap 3 being filled with a different batch of 0.0125M barium hydroxide ** The biodegradation process was terminated following Day 28 analysis and trap change; all traps remaining were taken for analysis on Day 29
Cumulative Biodegradation (%)
Test Item: m/p DIOL
Reference Item:Sodium Benzoate
Toxicity ControlTest and Reference Items
* A trap change was conducted on Day 28, after which the biodegradation process was terminated and final trap analysis conducted on Day 29. The tabulated result represents the sum of all analyses
** Mean cumulative biodegradation for Test Bioreactors = 2.0%
The cumulative biodegradation determined in the Toxicity Control bioreactor is expressed in terms of the total organic carbon content resulting from the reference item only (A) and for the total organic carbon resulting from the reference and test items (B).
The ready biodegradability of the Reaction Mass of α,α,α’,α’-Tetramethyl-m-xylene-α,α’-diol and α,α,α’,α’ -Tetramethyl-p-xylene-α,α’-diol: (m/p DIOL) was assessed over a 28 day period by the CO2Evolution (Modified Sturm) Test. The study was conducted in accordance with procedures outlined in OECD Guideline 301B (1992).
The results of the test were as follows (28 day percent biodegradation values):
*The cumulative biodegradation determined in the Toxicity Control bioreactor is expressed in terms of the total organic carbon content resulting from the reference item only (A) and for the total organic carbon resulting from the reference and test item (B).
The test item (The Reaction Mass of a,a,a’,a’-Tetramethyl-m-xylene-a,a’-diol and a,a,a’,a’ -Tetramethyl-p-xylene-a,a’-diol: (m/p DIOL) was not readily biodegradable under the conditions of the test as it failed to achieve a transition from 10% to 60% degradation in a 10 day window during the 28 day test. The test item was not considered to be inhibitory to the microbial inoculum since the biodegradation observed in the toxicity control bioreactor was similar to that expected from the individual test and reference bioreactors.
The test failed to meet the validity criterion with respect to the differences in extremes of percent biodegradation in the test bioreactors. However, this was judged to be due to very low biodegradation values observed (1.8 and 2.2% for Test Bioreactors 1 and 2 respectively) and the possible influx of atmospheric CO2 into the final trap on Day 29. The test is therefore considered valid.
α,α,α',α'-tetramethyl-m-xylene-α,α'-diol (m-diol) is considered not readily biodegradable.
α,α,α',α'-tetramethyl-m-xylene-α,α'-diol (m-Diol) is considered not readily biodegradable based on ready and inherent biodegradability studies, as well as a
valid study according to OECD 301B and GLP with the Reaction Mass of α, α, α’, α’-Tetramethyl-m-xylene-α, α’-diol and α, α, α’, α’ -Tetramethyl-p-xylene- α, α’-diol (m/p Diol).
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