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In this section only data from the parent compound SIKA Hardener LI are presented. For a detailed summary of the chosen Weight of evidence approach, please refer to the IUCLID5 endpoint summaries on acute toxicity of fish, dpahina and algae.

Short-term toxicity to fish

SIKA Hardener LI was assessed in a short-term toxicity to fish study, according to EU method C.1 and OECD guideline 203. Young fish were exposed in a semi-static test to the test item for 96 hours to water accomodated fractions (WAF) with nominal concentrations of 6.25; 12.5; 25.0; 50.0 and 100.0 mg/L. The 96 h LC50 was determined to be 87.2 mg/L and the 96 h NOEC was determined to be 25.0 mg/L.

Short-term toxicity to aquatic invertebrates

SIKA Hardener LI was assessed in a short-term toxicity to aquatic invertebrates study according to EU-method C.2 and OECD guideline 202. Young Daphnia were exposed in a semi-static test to water accomodated fractions (WAF) at nominal concentrations of 100 mg/L. The 48 h EC50 was determined at greater than 100 mg/L and the 48 h NOEC at 100 mg/L.

Toxicity to aquatic algae and cyanobacteria

SIKA Hardener LI was assessed in an aquatic toxicty test to unicellular green algal (Pseudokirchneriella subcapitata) according EU-method C.3 and OECD guideline 201. A water accommodated fraction (WAF) of 100 mg/L was prepared and nominal concentrations of up to 100 mg/L tested. The 72-h EC50 based on growth rate was extrapolated at 180.4 mg/L. The overall NOEC was determined to be 25.0 mg/L.

Toxicity to microorganisms

SIKA Hardener LI was assessed in an activated sludge respiration inhibition test, according to the EU-method C. 11 and OECD guideline 209. The 3-hour EC50 was determined to be 564.94 mg/L and the 3-hour NOEC determined to be 31 mg/L.