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Long-term toxicity to aquatic invertebrates

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Description of key information

A semi-static study according to OECD 211 was performed with 2,2'-azobis[2-methylbutyronitrile] to assess the chronic toxicity of the test item to Daphnia magna. Daphnia magna were exposed (10 replicates of a single daphnid per group) to a solution of the test item at a single nominal concentration of 10 mg/L for a period of 21 days. The geometric mean measured test concentrations were determined to be 8.4 mg/L and no effects were found at this concentration. The 21d-NOEC is therefore ≥ 8.4 mg/L.


The 21-day NOEC and LOEC in a semi-static study with Daphnia magna for 2,2’-azobis(isobutyronitrile), based on cumulative number of juveniles produced were 2.2 and 4.6 mg/L, respectively. Converted with molecular weight to 2,2'-azobis[2-methylbutyronitrile] the NOEC becomes 2.58 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
8.4 mg/L

Additional information