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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1993
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Short abstract from the 20th Annual meeting of the Japanese Society of Toxicological Sciences. No study details provided.

Data source

Reference
Reference Type:
publication
Title:
MUTAGENICITY OF AZO COMPOUNDS
Author:
Takenaka, S.
Year:
1993
Bibliographic source:
20TH ANNUAL MEETING OF THE JAPANESE SOCIETY OF TOXICOLOGICAL SCIENCES, CHIBA, JAPAN, JULY 29-30, 1993. JOURNAL OF TOXICOLOGICAL SCIENCES; 18 (4). 1993. 418.
Report Date:
1993

Materials and methods

Principles of method if other than guideline:
The micronucleus test using acridine orange staining method was performed in male ddY mice (8-week old) following double oral administration.
GLP compliance:
not specified
Type of assay:
mammalian erythrocyte micronucleus test

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
mouse
Strain:
other: male ddY mice
Details on species / strain selection:
The micronucleus test using acridine orange staining method was performed in male ddY mice (8-week old) following double oral administration.
Sex:
male

Administration / exposure

Route of administration:
oral: unspecified

Results and discussion

Test results
Sex:
male
Genotoxicity:
negative
Remarks on result:
other: see Remarks
Remarks:
2,2'-azobis (2-methylbutyronitrile) did not produce significant increase in the frequency of micronucleated polychromatic erythrocytes in the bone marrow of the treated mice.

Applicant's summary and conclusion

Conclusions:
2,2'-azobis(2-methylbutyronitrile) did not produce significant increase in the frequency of micronucleated polychromatic erythrocytes in
the bone marrow of the treated mice.
Executive summary:

The micronucleus test using acridine orange staining method was performed in male ddY mice (8-week old) following double oral administration.

2,2'-azobis(2-methylbutyronitrile) did not produce significant increase in the frequency of micronucleated polychromatic erythrocytes in the bone marrow of the treated mice.