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EC number: 236-740-8
CAS number: 13472-08-7
study was conducted to determine the effect of the test substance on the
growth and growth rate of the green alga, Selenastrum
capricornutum. The algae were exposed to nominal concentrations of
6.25, 12.5, 25, 50, and 100 mg/L of nutrient medium. For
the definitive (dose-response) test, the organisms were exposed for 72
hours (3 days) without test medium renewal. The
effect was expressed as percent inhibition in growth based on healthy
cell count (cell density), area under the growth curve, and growth rate
relative to the blank (normal culture medium) control for the 72-hour
(day 3) interval of the test. The
72-hour results, including the 95% confidence interval (C.I.), based on
nominal concentrations are as follows: Healthy Cell Count EC50 of
38.1 mg/L (95% C.I. = 32.6 to 44.6 mg/L) and NOEC of 12.5 mg/L; Area
Under the Growth Curve EC50 of
31.3 mg/L (95% C.I. = 23.6 to 39.1 mg/L) and NOEC of 12.5 mg/L; Growth
Rate EC50 of
67.0 mg/L (95% C.I. = 60.5 to 74.1 mg/L) and NOEC of 12.5 mg/L. The
ability of the organisms to recover was assessed for each definitive
test concentration with 50% or greater growth inhibition (i.e., nominal
50 and 100 mg/L). The effects on growth and growth rate of Selenastrum
found to be algistatic, i.e., logarithmic growth resumed within 144
hours (6 days), at concentrations less than or equal to 100 mg/L.
static, 72-hour study in Selenastrum
capricornutum with the test substance was
performed. The 72-hour EC50 in Selenastrum
capricornutum was 67 mg/L (measured initial) based on
growth rate. The NOEC based on growth rate was 12.5 mg/L (measured
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