Sodium 2,4,5-trichlorobenzenesulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 Feb 18 - 17 April 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

CAS#: 53423-65-7
EC#: 258-548-3
Label Identification: Arichem, LLC; 187 Sloss Industries Road; Ariton, AL 36311 USA
Product Sample: Sodium Trichlorobenzene Sulfonate
Lot: 1708-09; Approx 100g
Date & Quantity Received: 29 Jan 18; 109.7 g (GW)
Physical Description: White powder
Storage Conditions: Room temperature
Purity: 100% active ingredient, refer to the Sponsor provided Certificate of Analysis

Analytical monitoring:

no

Remarks:

Substance is a non-volatile solid and known to be stable in water at environmetal pH

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Test Room: Environmentally controlled chamber A
Test Chambers: 250 mL glass beakers; filled with 200 mL of the appropriate solution
Test Medium: Moderately hard synthetic freshwater:
Holding: Before dosing, the daphnids were held in water of the quality used in the test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

Range-finding: 215 mg/L CaCO3
Limit Test: 180 mg/L CaCO3

Test temperature:

Target: 20 ± 1°C
Actual: 21 °C

pH:

Range-finding: 7.8
Limit Test: 7.4

Dissolved oxygen:

Range-finding: 83-93%
Limit Test: 91-95%

Conductivity:

652 - 687 µmhos/cm

Nominal and measured concentrations:

Range-finding: 0.01 mg/L, 0.1 mg/L, 1.0 mg/L, 10 mg/L, and 100 mg/L
Limit Test: 100 mg/L

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

All organisms were mobile at the end of the study and thus the EC50 was determined to be >100 mg/L.

Limit Test Survival:

Conc. (mg/L)	Replicate	Number of Surviving Organisms			Survival
		0 Hours	24 Hours	48 Hours
Control	A	5	5	5	100%
	B	5	5	5
	C	5	5	5
	D	5	5	5
100	A	5	5	5	100%
	B	5	5	5
	C	5	5	5
	D	5	5	5

Note:All organisms had no observable abnormalities unless otherwise indicated.

Validity criteria fulfilled:

yes

Conclusions:

The EC50 was determined to be >100 mg/L.

Executive summary:

In a GLP guidline study conducted according to OECD 202, the substance was evaluated for toxicity to Daphnia Magna in a 48-hour static test at a limit dose of 100 mg/L. All test organisms (100%) survived the 48 -hour test period. Given these results, the EC50 was determined to be >100 mg/L.

Description of key information

In a GLP guidline study conducted according to OECD 202, the substance was evaluated for toxicity toDaphnia Magnain a 48-hour static test at a limit dose of 100 mg/L. All test organisms (100%) survived the 48 -hour test period. Given these results, the EC50 was determined to be >100 mg/L.

Additional information

The EC50 was determined to be >100 mg/L.