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EC number: 209-060-4 | CAS number: 554-12-1
The 24-hour LC50 of 173 substances hazardous to water was determined be means of a standardised procedure using 24-hour animals from a clone of Daphnia magna. At the same time, the LC0 and LC100 for each of these pollutants were determined. The test medium was tap water free from chlorine, saturated with oxygen.
Under the conditions of the study the 24-hour LC50 of the registered substance to the aqautic invertebrate, Daphnia magna, was determined to be 500 mg/L. The 24-hour LC0 and LC100 were determined to be 63 mg/L and 1000 mg/L, respectively.
The toxicity of 183 substances to the aquatic invertebrate, Daphnia magna, was assessed in defined standardised culture medium (artificial freshwater) according to a standardised procedure. The Daphnia were exposed to the test media for 24 hours and the number of immobilised daphnia were counted. The 24 hour EC0, EC50 and EC100 were determined.
Under the conditions of the study the 24-hour EC50 of the test material to the aqautic invertebrate, Daphnia magna, was determined to be 1000 mg/L. The 24-hour EC0 and EC100 were determined to be 4-120 mg/L and 1786 mg/L, respectively.
A publication reporting on investigations carried out in order to determine the "no observed effect concentration" (NOEC) of 73 environmentally relevant substances in the 21 d Daphnia reproduction test also presents results from an acute 24 h Daphnia test and a preliminary 3 d Daphnia test (same conditions as in the 21 d reproduction test). However, no information is provided on the test methods of the acute 24 hour test and no results are presented from the 3 d preliminary study.
The publication reports the 24 hour EC50 for the aquatic invertebrate, Daphnia magna, to be 516 mg/L.
Three publications, including information on the acute toxicity of the registered substance, to the aquatic invertebrate, Daphnia magna, are available. Two publications (Bringmann 1977 and Bringmann 1982) include well documented study methodology which is acceptable for assessment of the relevant results presented. These two references were therefore awarded a reliability score of 2 in accordance with the criteria set forth by Klimisch et al. (1997). The Kuhn (1989) reference did not contain information on the methodology used for determining the 24-hour EC50 value reported and was therefore awarded a reliability score of 4 in accordance with the criteria set forth by Klimisch et al. (1997).
The three references report comparable results and are therefore considered together in a weight of evidence approach. The lowest EC50 value reported is taken as the key value as this is deemed to be the most conservative approach.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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