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EC number: 209-060-4 | CAS number: 554-12-1
Table 1: Summary of Results from Experiment 1
Mean Number of Revertant Colonies Per Plate in the Absence of S9 Mix
Dose level (µg per plate)
Mean Number of Revertant Colonies Per Plate in the Presence of S9 Mix
* Results from re-tests
NaN3: sodium azide
Table 2: Summary of results from Experiment 2
* Results from re-test
The mutagenic potential of the test material was investigated in a study which was conducted in accordance with the standardised guidelines OECD 471 and EU Method B.13/14, under GLP conditions.
During the study the test material was tested for mutagenic activity in Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 and Escherichia coli WP2uvrA at concentrations ranging from 17 to 5000 μg per plate. The solvent used was sterile, ultra-pure water.
Two independent tests (one direct plate and one pre-incubation) were conducted on agar plates in the presence and absence of an Aroclor-1254 induced rat liver preparation and the co-factors required for mixed-function oxidase activity (S9 mix). The first mutation assay was repeated in the presence of S9 mix only due to an operator error.
Concurrent positive controls demonstrated the sensitivity of the assay and the metabolising activity of the S9 mix.
No mutagenic activity was observed in any of the 5 bacterial strains, in either activation condition.
There was no toxicity to the bacteria and no precipitation of the test material was observed in either mutation assay, in either the presence or the absence of S9 mix.
It was concluded that the test material was not mutagenic to Salmonella typhimurium or Escherichia coli when tested in sterile, ultra-pure water up to a pre-determined maximum concentration of 5000 μg per plate.
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