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Hydrolysis

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Reference
Endpoint:
hydrolysis
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 111 (Hydrolysis as a Function of pH)
GLP compliance:
no
Remarks:
In house study in non-GLP facility
Analytical monitoring:
yes
Buffers:
the solutions contained 0.005M KHP as a buffer
Details on test conditions:
A jacketed beaker filled with DI water was heated and maintained at 37°C using an immersion heater and pump. The immersion heater was placed in a 3-gallon reservoir of water and controlled using the temperature control unit. The thermocouple was suspended in the jacketed beaker next to the sample being hydrolyzed, to ensure the temperature the sample was exposed. Samples were taken from the sample vial thru the septa using a clean and dried stainless-steel needle at desired time intervals and at time zero.

The sample aliquots removed (1mL) from the reaction mixture were analyzed using gas chromatography. An internal procedure was used to calibrate the GC to correlate the weight percent in each sample compared to peak area from the eluted peak in the chromatogram; the internal standard used was acetone. The analysis conducted targets the degradation of the 2-ethyl-oxazoline through a hydrolytic process at varying pH, and does not monitor the formation of the hydrolyzed products.
Duration:
6.5 h
pH:
2.98
Temp.:
37 °C
Duration:
6.33 h
pH:
6.98
Temp.:
37 °C
Duration:
6.3 h
pH:
10
Temp.:
37 °C
Transformation products:
not measured
Key result
pH:
2.98
Temp.:
37 °C
Hydrolysis rate constant:
0.103 h-1
DT50:
6.73 h
Type:
(pseudo-)first order (= half-life)
Key result
pH:
6.98
Temp.:
37 °C
Hydrolysis rate constant:
0.167 h-1
DT50:
4.14 h
Type:
(pseudo-)first order (= half-life)
Key result
pH:
10
Temp.:
37 °C
Hydrolysis rate constant:
0.052 h-1
DT50:
13.36 h
Type:
(pseudo-)first order (= half-life)
Validity criteria fulfilled:
not specified
Conclusions:
The test substance undergoes hydrolysis in contact with water.
At pH 2.98 T1/2 = 6.73 hours
At pH 6.98 T1/2 = 4.14 hours
At pH 10.00 T1/2 = 13.36 hours

Description of key information

The test substance undergoes hydrolysis in contact with water.

At pH 2.98 T1/2 = 6.73 hours

At pH 6.98 T1/2 = 4.14 hours

At pH 10.00 T1/2 = 13.36 hours

Key value for chemical safety assessment

Half-life for hydrolysis:
4.14 h
at the temperature of:
37 °C

Additional information

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