2-ethyl-2-oxazoline

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Peer reviewed article

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

test concentrations only up to 2000 µg/plate

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

1

Reference substance name:

2-aminoethanol

EC Number:

205-483-3

EC Name:

2-aminoethanol

Cas Number:

141-43-5

Molecular formula:

C2H7NO

IUPAC Name:

2-aminoethanol

Specific details on test material used for the study:

- Name of test material (as cited in journal article): Monoethanolamine
- Analytical purity: no data
- Batch No.: 17589

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium, other: TA 1535, TA 1537, TA1538, TA 98 and TA 100

Details on mammalian cell type (if applicable):

- Source: obtained from Professor B.N. Ames, University of California

Species / strain 2

Species / strain / cell type:

E. coli, other: WP2 an WP2 uvrA

Details on mammalian cell type (if applicable):

- Source: obtained from Dr. M.H.L. Green, University of Sussex

Metabolic activation:

with and without

Metabolic activation system:

rat liver S9 (Arcoclor 1254 induced)

Test concentrations with justification for top dose:

0.2, 2, 20, 500 and 2000 μg/plate

Vehicle / solvent:

water

Controls

Untreated negative controls:

not specified

Positive controls:

yes

Positive control substance:

4-nitroquinoline-N-oxide

7,12-dimethylbenzanthracene

sodium azide

benzo(a)pyrene

cyclophosphamide

ethylmethanesulphonate

methylmethanesulfonate

other: neutral red

Details on test system and experimental conditions:

METHOD OF APPLICATION:
- plate-incorporation assay: in medium; in agar; according to method described by Ames et al. (1975)
- pre-incubation assay: in agar, according to method described by Brooks and Dean (1981)
- spot test: test compound added to a 1-cm diameter sterile filter disc before adding to the centre of the seeded plate; according to method described by Ames et al., (1975)
- treat-and-plate method: overnight broth cultures

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium, other: TA 98, TA 100, TA 1535, TA 1537, TA 1538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Positive controls validity:

not specified

Test results 2

Species / strain:

E. coli, other: WP2 an WP2 uvrA

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Positive controls validity:

not specified

Applicant's summary and conclusion

Conclusions:

The in vitro genotoxic potential of 2-aminoethanol in bacteria was investigated in two species: (1) Salmonella typhimurium TA1535, TA1537, TA1538, TA98 and TA100; and (2) Escherichia coli WP2 and WP2 uvrA. In both species, negative results were reported for 2-aminoethanol with and without activation.