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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Method similar to guideline but no analytical, and test species is not the standard species
Justification for type of information:
Read-across from supporting substance (structural analogue or surrogate)
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
no chemical analysis; Goldorfen is not the standard test species but is among those often tested
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
diluted in water only
Test organisms (species):
Leuciscus idus melanotus
Details on test organisms:
TEST ORGANISM
- Common name: Goldorfen
- Source: Paul Eggers, 2345 Hohenwestedt
- Age at study initiation (mean and range, SD): not indicated
- Length at study initiation (length definition, mean, range and SD): 5.5 - 6.6 cm
- Weight at study initiation (mean and range, SD): 1.5-2.7 g
- Feeding during test: no

ACCLIMATION
- Acclimation period: no indicted
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min
- Feeding frequency: not indicated
- Health during acclimation (any mortality observed): not indicated

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
114 mg CaCO3/L
Test temperature:
20 +/- 1 C
pH:
7.5 - 8.2 (except at 500 mg/L where it was 5.7)
Dissolved oxygen:
8.5 - 9.2 mg/L
Salinity:
N/A
Nominal and measured concentrations:
Nominal - 0, 10, 100 500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquarium with 20L solution
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 20L
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: not indicated

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
- Ca/mg ratio: 0.7
- Na/K ratio: 21
- Intervals of water quality measurement: 0, 2, 24, 48, 72 and 96 hours

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12
- Light intensity: 700 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality daily; observations regularly

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 5-10X
- Range finding study: not indicated
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
>325 mg/L based on active ingredient
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: no difference compared to control group
- Observations on body length and weight: not indicated
- Other biological observations: macroscopic examination showed no changes
- Mortality of control: none
Validity criteria fulfilled:
yes
Conclusions:
Test substance was not toxic at 500 mg/L (325 mg/L active substance), the highest concentration tested.

Description of key information

LC50 for freshwater fish 96h= 325 mg/L

Key value for chemical safety assessment

LC50 for freshwater fish:
325 mg/L

Additional information

A single, good quality (non-GLP) fish acute toxicity study is available for p-toluenesulfonic acid (CAS No. 104-15-4). The 96 hr LC50 is 500 mg/L which is equivalent to 325 mg active ingredient/L based on the test substance being 65% p-toluenesulfonic acid in water.

The study was conducted in 1981 with a test substance with a 65% purity (in solution). The testing procedure was similar to OECD Guideline 203 except there was no analytical confirmation of the test concentration, and the test species was the Goldorfen which, while often used, is not a standard test species. The study was a 96 hour, static exposure to 10, 100 and 500 mg/L test substance. The test conditions were confirmed as valid. No mortality or behavior abnormalities were obsered at any of the exposure levels or in the controls.No toxicity was observed in the study. The 96 hr LC50 (and NOEC) are 500 mg/L which is equivalent to 325 mg active ingredient/L based on the test substance being 65% p-toluenesulfonic acid in water.