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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1962
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Not a guideline study and not all details available
Justification for type of information:
Grouping of structurally similar substances (category approach)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1962

Materials and methods

Principles of method if other than guideline:
9 animals were exposed to 0.1 mL of the test substance for 5, 10 or 30 seconds (3 animals for each duration) and then eyes were rinsed. The Draize, Woodard and Calvery scoring system was used. Observations were at 1, 4, 24, 48, 72, 120 and 168 hours following exposure. Reversibility was determined up to 14 days post exposure.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
organic liquid at 65% purity

Test animals / tissue source

Species:
rabbit
Strain:
not specified

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not specified
Duration of treatment / exposure:
5, 10 or 30 seconds
Observation period (in vivo):
168 hours for scoring and 14 days for reversibility
Number of animals or in vitro replicates:
3 at each treatment duration

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 5 second exposure
Score:
>= 83.6 - < 110
Max. score:
110
Reversibility:
not fully reversible within: 14 days
Remarks on result:
other: overall irritation score was 83.6 out of 110 at 5 second exposure, and 110 out of 110 at both 10 and 30 second exposures
Other effects:
With 5 second exposure there was copious discharge, swelling with lids about half closed, severe redness of conjunctivae, and opacity sufficient to render the iris invisible, all developed within a few minutes. After 14 days there is moderate edema and discharge, moderately severe erythema, and opalescent areas. The iris is reacting to light.
With 10 second exposure, the response was similar to 5 seconds except the iris seems to be destroyed at 14 days. Maximum score of 110 reached in 4 hours.
With 30 second exposure, the response was similar to 10 seconds but with the area of severe edema and congestion extending for a greater distance around the eye.
The cornea became opalescent in 4-8 seconds and the swelling was rapid in all exposures.

Applicant's summary and conclusion

Interpretation of results:
corrosive
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The test substance is corrosive to the eye even with short (i.e., 5-30 second) exposure.