Registration Dossier
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EC number: 619-171-3 | CAS number: 95759-44-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Dec 12, 2006 - Feb 27, 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- July, 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Purity: 99.9 %
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was insufficiently soluble to permit addition via aqueous solution. Individually weighed quantities of test substance were added directly to the appropriate test vessels.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge, micro organisms from a domestic waste water treatment plant was supplied by the sewage plant Darmstadt, Germany.
Preparation and Maintenance
Tue activated sludge was washed by centrifugation and the supernatant liquid phase was decanted. Tue solid material
was re-suspended in tap water and again centrifuged. The latter procedure was repeated twice. The washed sludge was re-suspended in tap water and sewage feed ( 50 mL per L) was added. The sludge was kept at room temperature under continuous aeration until use for one day.
Immediately before use, the dry weight of the activated sludge was detennined, and diluted to 3.5 g/L with tap water. Tue pH of the activated sludge was 7.9 and therefore no adjustment
was necessary. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2°C
- pH:
- 7.01 - 7.25
- Dissolved oxygen:
- saturated
- Nominal and measured concentrations:
- 10, 32, 100, 320 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: ca 250 mL glass conical flask
- Type (delete if not applicable): open
- Aeration:compressed air supply
- No. of vessels per control (replicates): 1
- Sludge concentration (weight of dry solids per volume): 3 g/L
- Nutrients provided for bacteria: no
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Particulate matter:
OTHER TEST CONDITIONS
- Adjustment of pH: yes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Justification for using fewer concentrations than requested by guideline: n.a.
- Range finding study no
- Test concentrations: 10, 32, 100, 320, 1000 mg/L - Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- 3,5-DCP:
Total Respiration: 8.2 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the measured inhibition of respiration rates, neither EC20 nor EC50 could be calculated. Since the inhibition of respiration rates was clearly below 20% up
to and including the concentration of 1000 mg/L, the EC20 and EC50 can be assumed to be higher than 1000 mg/L. A NOEC can be established at 1000 mg/L since the respiration inhibition was clearly lower than 20% at this concentration. - Executive summary:
The informtion for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 209.
Based on the measured inhibition of respiration rates, neither EC20 nor EC50 could be calculated. Since the inhibition of respiration rates was clearly below 20% up
to and including the concentration of 1000 mg/L, the EC20 and EC50 can be assumed to be higher than 1000 mg/L. A NOEC can be established at 1000 mg/L since the respiration inhibition was clearly lower than 20% at this concentration.
Reference
Description of key information
OECD 209 study (Reliability 1): NOEC = 1000 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
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