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Ecotoxicological information

Long-term toxicity to fish

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Reference
Endpoint:
adult fish: sub(lethal) effects
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2006-08-21 to 2006-11-15
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study performed according to OECD 305 and METI Guideline: Concentration Test on Chemical Substances in Fish.
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
Qualifier:
according to
Guideline:
other: METI Guideline: Concentration Test on Chemical Substances in Fish
GLP compliance:
yes (incl. certificate)
Test material information:
Composition 1
Specific details on test material used for the study:
Puritiy: > 99.0 %
Analytical monitoring:
yes
Details on sampling:
- Sampling intervals/frequency for test organisms: days 0, 5, 8, 14, 21, 28
- Sampling intervals/frequency for test medium samples: days 0, 5, 8, 14, 21, 28
Vehicle:
yes
Details on test solutions:
According to guideline

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)): 12 mg/l

Dose 1: Test item: 0.2 mg/L ,
Dose 2: Test item: 0.02 mg/L
Test organisms (species):
Cyprinus carpio
Details on test organisms:
Cyprinus carpio
Lot: 060710c
Weight: 5.8 g
Length: 7.3 cm

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
28 d
Hardness:
250 g/L
Test temperature:
25.0 ° +/- 0.4 °C
pH:
7
Dissolved oxygen:
saturated
Nominal and measured concentrations:
nominal concentration 0.20 mg/L:
Solubilizer used: HCO-40, 12 mg/L

analytically measured concentrations:
day 4: 0.200 mg/L
day 7: 0.195 mg/L;
day 14: 0.196 mg/L
day 21: 0.198 mg/L
day 28: 0.201 mg/L


nominal concentration 0.02 mg/L
Solubilizer used: HCO-40, 12 g/L
analytically measured concentrations:
day 4: 0.0200 mg/L
day 7: 0.0199mg/L;
day 14: 0.0198 mg/L
day 21: 0.0200mg/L
day 28: 0.0200 mg/L

Details on test conditions:
After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 0, 5, 14, 21, and 28. The analytical values show that the nominal concentrations of 0.2 and 0.02 mg/L were maintained during the experimental phase.


Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
0.2 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Concentrations maintained using HCO-40
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
0.2 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Concentrations maintained using HCO-40
Validity criteria fulfilled:
yes
Conclusions:
The test material showed no toxic potential at concentrations at or above the water solubility. The NOEC was 0.2 mg/L and thus higher than the limit of water solubility.
Executive summary:

The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions. During the GLP compliant test the lethal and other effects in fish were determined during the exposure period of 28 days.

The test material showed no toxic potential at concentrations at or above the water solubility. The NOEC was 0.2 mg/L and thus higher than the limit of water solubility.

Description of key information

Data from a GLP study according to OECD TG 305 are available.

NOEC (28 d) = 0.20 mg/L (12 mg/L HCO-40)

LC50 (28 d)  > 0.20 mg/L (12 mg/L HCO-40)

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater fish:
0.2 mg/L

Additional information

NOEC (BCF, 28d) > 0.20 mg/L