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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
04/05/2000 - 03/08/2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study according to international guideline (ISO Standard 6341) under GLP, validity criteria met.
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
ISO 6341 15 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Deviations:
yes
Remarks:
protected from light
Principles of method if other than guideline:
Not relevant
GLP compliance:
yes
Remarks:
Statement of Compliance

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Dihydrolinalool
- Physical state: Liquid
- Storage condition of test material: In refrigerator, protected from light
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not relevant

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: Nominal: 5.6, 10, 18, 32 and 56 mg/l
- Sampling method: 10 ml from the approximate centre of the test vessel from freshly prepared solutions of 5.6, 18 and 56 mg/l and from 24h-old
solutions of 5.6, 18 and 56 mg/l. Additionally, reserve samples of 10 ml were taken. These samples were stored in a deep-freeze for possible analysis until delivery of the final report with a maximum of three months.
- Sample storage conditions before analysis: Not applicable, samples were analysed on the day of
sampling.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of test solutions started with a solution of 100 mg/l (range-finding test) or a stock solution of 1000 mg/I (final test). Magnetic stirring was sufficient to achieve complete dissolution of the test substance in the test medium. The lower concentrations tested were prepared by
subsequent dilutions in test medium.
- Controls: yes, test medium without test substance or other additives.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The batch of DIHYDROLINALOOL tested was a clear and colourless liquid and
was completely soluble in test medium at the concentrations tested.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus 1820
- Age at study initiation (mean and range, SD): <24 hours
- Method of breeding: Breeding starts with new-born animals, less than 3 days old, placing about 250 of them into 10 liters of medium in an all-glass culture vessel. After 7 days of cultivation half of the medium is renewed twice a week. Cultures are kept at 18 - 22 degrees Celsius.
- Feeding during test: No


ACCLIMATION
- Acclimation period: 24 hours
- Acclimation conditions (same as test or not): Yes

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
Not relevant

Test conditions

Hardness:
250 mg/l CaCO3
Test temperature:
20.5 - 21.2 °C in blank control.
pH:
7.8 - 8.0
Dissolved oxygen:
8.0 - 9.3 mg/l
Salinity:
Not relevant
Nominal and measured concentrations:
Nominal: 5.6, 10, 18, 32 and 56 mg/l
Measured (0 h): 6.30, 20.2 and 57.8 mg/l
Measured (24 h, old): 5.56, 17.9 and 54.7 mg/l
Measured (24 h, old): 5.68, 17.8 and 57.9 mg/l
Concentrations were only measured in test vessels with nominal concentrations of 5.6, 18 and 56 mg/l.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: all-glass 100 ml, filled with 80 ml medium
- Aeration: None
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Milli-RO water containing: 293.8 mg/l CaCl2.2H2O, 123.3 mg/l MgSO4.7H2O, 64.8 mg/l NaHCO3, 5.8 mg/l
KCl, 0.125 mg/l B, 0.05 mg/l Fe, 0.025 mg/l Mn, 0.0125 mg/l Li, Rb and Sr, 0.0063 mg/l Mo, 0.0025 mg/l Br, 0.0016 mg/l Cu, 0.0063 mg/l Zn, 0.0025 mg/l Co and J, 0.0010 mg/l Se, 0.0003 mg/l V, 2.5 mg/l Na2EDTA.2H2O, 10.0 mg/l Na2SiO3.9H2O, 0.27 mg/l NaNO3, 0.14 mg/l KH2PO4, 0.18 mg/l K2HPO4, 75.0 ug/l Thiamine, 1.0 mg/l B12 and 0.75 ug/l Biotin.
- Culture medium different from test medium: No
- Intervals of water quality measurement: 24 hours


OTHER TEST CONDITIONS
- Photoperiod: Continuous dark
- Light intensity: 12 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobilization


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95%CL: 29 - 37 mg/l
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
56 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: no immobilisations in the control treatments
- Effect concentrations exceeding solubility of substance in test medium: DIHYDROLINALOOL was completely soluble in test medium at the
concentrations tested.
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Mortality: 0% in control, 100% at 1 mg/l after 48 hours.
- EC50/LC50: the 48h-EC50 was 0.60 mg/l with 95% fiducial limits of 0.52 - 0.79 mg/l.
Reported statistics and error estimates:
The EC50-value was calculated at 48 hours of exposure from the probits of the percentages of affected daphnia and the logarithms of the
corresponding test substance concentrations using the maximum likelihood estimation method (Finney, D.J 1971: Probit analysis, Cambridge University Press, Cambridge, U.K., 3rd edition).

Any other information on results incl. tables

not relevant

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Control immobilisation <10%; oxygen contentration remained > 2 mg/l, 24h-EC50 of potassium dichromate within range
Conclusions:
The 48h-EC50 of Dihydrolinalool to Daphnia magna is 32 mg/l.
Executive summary:

The toxicity of Dihydrolinalool towards Daphnia magna was investigated according to ISO Guideline 6341. The 48h-EC50 was found to be 32 mg/l (29 - 37 mg/l), the 24h EC50 was found to be 56 mg/l. The 48h NOEC is 18 mg/l.