3-aminobenzenesulphonic acid

Repeated dose toxicity: dermal

Administrative data

Endpoint:

short-term repeated dose toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Predates implementation of GLP and/or development of study guidelines, available as unpublished report, limitations in design and/or reporting acceptable with restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Six albino rabbits were used, the hair was clipped from the back of each of these animals. The test material was applied as 40% suspension in 1% aqueous carboxymethyl cellulose to each of three rabbits with intact skin and to each of three rabbits with abraided skin. The volume of the test material applied was approximately 2.0 mL per rabbit, which was spread and rubbed evenly onto the skin and into the abrasions once a day on five days of the week, over a period of 14 days.

GLP compliance:

not specified

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-aminobenzenesulphonic acid

EC Number:

204-473-6

EC Name:

3-aminobenzenesulphonic acid

Cas Number:

121-47-1

Molecular formula:

C6H7NO3S

IUPAC Name:

3-aminobenzene-1-sulfonic acid

Details on test material:

- Name of test material (as cited in study report): Metanilic Acid Cake, Ba 38/76, TR 76-415 (M53740; UM 44)

Test animals

Species:

rabbit

Strain:

other: albino

Sex:

not specified

Details on test animals or test system and environmental conditions:

- Weight at study initiation: 1.89 - 2.11 kg

Administration / exposure

Type of coverage:

not specified

Vehicle:

other: 1% aqueous carboxymethyl cellulose

Details on exposure:

TEST SITE
- Area of exposure: the back
- Coverage: 12 x 18 cm per rabbit; covered approximately 10% of the total body surface of a rabbit
- Type of wrap if used: no data

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no data

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2.0 mL per rabbit
- Concentration (if solution): 40% suspension in 1% aqueous carboxymethyl cellulose.

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

14 days

Frequency of treatment:

Once a day on five days of the week.

Doses / concentrations

Remarks:

Doses / Concentrations:
40% suspension in 1% aqueous carboxymethyl cellulose
Basis:

No. of animals per sex per dose:

6 animals (3 with intact skin, 3 with abraided skin)

Control animals:

not specified

Details on study design:

no data

Examinations

Observations and examinations performed and frequency:

DERMAL IRRITATION (if dermal study): Yes
BODY WEIGHT: Yes
CAGE SIDE OBSERVATIONS: Yes
URINALYSIS: Yes

Sacrifice and pathology:

no data

Results and discussion

Results of examinations

Details on results:

The compound produced no signs of local irritation. The abraided areas had healed nearly completely at the end of the two-week period. The gain in body weight of rabbits was above normal. (average body weight gain intact skin group 0.73 kg, abraided skin group 0.61 kg). Compound is brown in color, and stained the skin and fur light brown. The urine of all animals had a brownish color, indicating that a portion of the compounds applied was absorbed. Signs of systemic intoxication were not noted.

Target system / organ toxicity

Critical effects observed:

not specified