3-aminobenzenesulphonic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 79/831 EWG (6), C2 of Annex V

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-aminobenzenesulphonic acid

EC Number:

204-473-6

EC Name:

3-aminobenzenesulphonic acid

Cas Number:

121-47-1

Molecular formula:

C6H7NO3S

IUPAC Name:

3-aminobenzene-1-sulfonic acid

Details on test material:

- Water solubility: > 500 mg/L at 20°C

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION OF TEST SOLUTION
- Concentration of test substance in stock solution: 500 mg/L
- Stock solution was stirred for 1 hour at 20°C

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Test organism: Daphnia magna STRAUS
- Origin: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978

CULTURING CONDITIONS
- Water properties: total hardness 2.7 +/- 0.5 mmol/L
molar ratio Ca:Mg ca. 4:1
oxygen > 2 mg7L
temperature 19 - 21°C
- Feeding: green algae daily and yeast at water renewal
- Illumination: day:night rhythm 16:8 hours

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19 - 21°C

pH:

3.89 - 8.1

Dissolved oxygen:

8.12 - 9.72 mg/L

Nominal and measured concentrations:

- Concentrations tested (nominal): 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: centrifugation vessels with flat bottom
- Test volume: 10 ml
- No. replicates/concentration: 4
- No. animals/replicate: 5
- No. animals/concentration: 20
- Test water: dechlorinated tap water
- Age of animals: 6 - 24 h
- Illumination: none

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

171.09 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility