Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report Date:
1987

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: 79/831 EWG (6), C2 of Annex V
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Water solubility: > 500 mg/L at 20°C

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION OF TEST SOLUTION
- Concentration of test substance in stock solution: 500 mg/L
- Stock solution was stirred for 1 hour at 20°C

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- Test organism: Daphnia magna STRAUS
- Origin: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978

CULTURING CONDITIONS
- Water properties: total hardness 2.7 +/- 0.5 mmol/L
molar ratio Ca:Mg ca. 4:1
oxygen > 2 mg7L
temperature 19 - 21°C
- Feeding: green algae daily and yeast at water renewal
- Illumination: day:night rhythm 16:8 hours

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
19 - 21°C
pH:
3.89 - 8.1
Dissolved oxygen:
8.12 - 9.72 mg/L
Nominal and measured concentrations:
- Concentrations tested (nominal): 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: centrifugation vessels with flat bottom
- Test volume: 10 ml
- No. replicates/concentration: 4
- No. animals/replicate: 5
- No. animals/concentration: 20
- Test water: dechlorinated tap water
- Age of animals: 6 - 24 h
- Illumination: none

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
125 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
171.09 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
250 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion