Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23.08.1976 - 23.09.1976
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report Date:
2012

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
not specified
Details on inoculum:
The details on the type and amount of inoculum have not been documented. However, the study director, Dr. H. Wellens was particitpant in the laboratory comparison tests for the development of the OECD test methods 301 and 302. For this reason it is most likely that the test conditions were already similar to the later OECD guideline 301E.
Duration of test (contact time):
32 d
Initial test substance concentration
Initial conc.:
48 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST SYSTEM
- Test vessel: 2 L Erlenmeyer flasks
- Treatment of test samples: filtrated through membrane filters, nominal diameter of the pores 0.2 µm
- Incubation of test vessels: testing was conducted on a rotary shaker with a diameter of the shaking circle of 5 cm and exposed to room temperature (20 - 22°C). The shaking frequency was set to about 70 - 100 rpm
Reference substance
Reference substance:
other: D(+)-glucose (monohydrate)

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
38
Sampling time:
26 d
Details on results:
- Degradation kinetics:
Time [d] Degradation [%]
1 0
2 26
12 0
16 18
26 38
30 42

BOD5 / COD results

Results with reference substance:
- Degradation (DOC-removal) of test substance after 12 days: 79 %

Applicant's summary and conclusion

Interpretation of results:
other: partly/moderately biodegradable